Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels

Jin Miyawaki, Sachiko Matsumura, Ryota Yuge, Tatsuya Murakami, Shigeo Sato, Akihiro Tomida, Takashi Tsuruo, Toshinari Ichihashi, Takako Fujinami, Hiroshi Irie, Kunihiro Tsuchida, Sumio Iijima, Kiyotaka Shiba, Masako Yudasaka

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Single-walled carbon nanohorns (SWNHs) are single-graphene tubules that have shown high potential for drug delivery systems. In drug delivery, it is essential to quantitatively determine biodistribution and ultrastructural localization. However, to date, these determinations have not been successfully achieved. In this report, we describe for the first time a method that can achieve these determinations. We embedded Gd2O3 nanoparticles within SWNH aggregates (Gd2O3@SWNHag) to facilitate detection and quantification. Gd2O3@SWNHag was intravenously injected into mice, and the quantities of Gd in the internal organs were measured by inductively coupled plasma atomic emission spectroscopy: 70-80% of the total injected material accumulated in liver. The high electron scattering ability of Gd allows detection with energy dispersive X-ray spectroscopy and facilitates the ultrastructural localization of individual Gd2O3@SWNHag with transmission electron microscopy. In the liver, we found that the Gd2O3@SWNHag was localized in Kupffer cells but were not observed in hepatocytes. In the Kupffer cells, most of the Gd2O3@SWNHag was detected inside phagosomes, but some were in another cytoplasmic compartment that was most likely the phagolysosome.

Original languageEnglish
Pages (from-to)1399-1406
Number of pages8
JournalACS Nano
Volume3
Issue number6
DOIs
Publication statusPublished - 23-06-2009

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Nanohorns
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Carbon
Nanoparticles
nanoparticles
carbon
liver
Liver
delivery
drugs
Atomic emission spectroscopy
Electron scattering
Graphite
Inductively coupled plasma
compartments
cells
Drug delivery
organs
Graphene
spectroscopy

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Miyawaki, J., Matsumura, S., Yuge, R., Murakami, T., Sato, S., Tomida, A., ... Yudasaka, M. (2009). Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels. ACS Nano, 3(6), 1399-1406. https://doi.org/10.1021/nn9004846
Miyawaki, Jin ; Matsumura, Sachiko ; Yuge, Ryota ; Murakami, Tatsuya ; Sato, Shigeo ; Tomida, Akihiro ; Tsuruo, Takashi ; Ichihashi, Toshinari ; Fujinami, Takako ; Irie, Hiroshi ; Tsuchida, Kunihiro ; Iijima, Sumio ; Shiba, Kiyotaka ; Yudasaka, Masako. / Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels. In: ACS Nano. 2009 ; Vol. 3, No. 6. pp. 1399-1406.
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abstract = "Single-walled carbon nanohorns (SWNHs) are single-graphene tubules that have shown high potential for drug delivery systems. In drug delivery, it is essential to quantitatively determine biodistribution and ultrastructural localization. However, to date, these determinations have not been successfully achieved. In this report, we describe for the first time a method that can achieve these determinations. We embedded Gd2O3 nanoparticles within SWNH aggregates (Gd2O3@SWNHag) to facilitate detection and quantification. Gd2O3@SWNHag was intravenously injected into mice, and the quantities of Gd in the internal organs were measured by inductively coupled plasma atomic emission spectroscopy: 70-80{\%} of the total injected material accumulated in liver. The high electron scattering ability of Gd allows detection with energy dispersive X-ray spectroscopy and facilitates the ultrastructural localization of individual Gd2O3@SWNHag with transmission electron microscopy. In the liver, we found that the Gd2O3@SWNHag was localized in Kupffer cells but were not observed in hepatocytes. In the Kupffer cells, most of the Gd2O3@SWNHag was detected inside phagosomes, but some were in another cytoplasmic compartment that was most likely the phagolysosome.",
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Miyawaki, J, Matsumura, S, Yuge, R, Murakami, T, Sato, S, Tomida, A, Tsuruo, T, Ichihashi, T, Fujinami, T, Irie, H, Tsuchida, K, Iijima, S, Shiba, K & Yudasaka, M 2009, 'Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels', ACS Nano, vol. 3, no. 6, pp. 1399-1406. https://doi.org/10.1021/nn9004846

Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels. / Miyawaki, Jin; Matsumura, Sachiko; Yuge, Ryota; Murakami, Tatsuya; Sato, Shigeo; Tomida, Akihiro; Tsuruo, Takashi; Ichihashi, Toshinari; Fujinami, Takako; Irie, Hiroshi; Tsuchida, Kunihiro; Iijima, Sumio; Shiba, Kiyotaka; Yudasaka, Masako.

In: ACS Nano, Vol. 3, No. 6, 23.06.2009, p. 1399-1406.

Research output: Contribution to journalArticle

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T1 - Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels

AU - Miyawaki, Jin

AU - Matsumura, Sachiko

AU - Yuge, Ryota

AU - Murakami, Tatsuya

AU - Sato, Shigeo

AU - Tomida, Akihiro

AU - Tsuruo, Takashi

AU - Ichihashi, Toshinari

AU - Fujinami, Takako

AU - Irie, Hiroshi

AU - Tsuchida, Kunihiro

AU - Iijima, Sumio

AU - Shiba, Kiyotaka

AU - Yudasaka, Masako

PY - 2009/6/23

Y1 - 2009/6/23

N2 - Single-walled carbon nanohorns (SWNHs) are single-graphene tubules that have shown high potential for drug delivery systems. In drug delivery, it is essential to quantitatively determine biodistribution and ultrastructural localization. However, to date, these determinations have not been successfully achieved. In this report, we describe for the first time a method that can achieve these determinations. We embedded Gd2O3 nanoparticles within SWNH aggregates (Gd2O3@SWNHag) to facilitate detection and quantification. Gd2O3@SWNHag was intravenously injected into mice, and the quantities of Gd in the internal organs were measured by inductively coupled plasma atomic emission spectroscopy: 70-80% of the total injected material accumulated in liver. The high electron scattering ability of Gd allows detection with energy dispersive X-ray spectroscopy and facilitates the ultrastructural localization of individual Gd2O3@SWNHag with transmission electron microscopy. In the liver, we found that the Gd2O3@SWNHag was localized in Kupffer cells but were not observed in hepatocytes. In the Kupffer cells, most of the Gd2O3@SWNHag was detected inside phagosomes, but some were in another cytoplasmic compartment that was most likely the phagolysosome.

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