Biological characteristics of subsilicone oil fluid and differences with other ocular humors

Hideyuki Shimizu, Hiroki Kaneko, Ayana Suzumura, Kei Takayama, Rina Namba, Yasuhito Funahashi, Keiko Kataoka, Takeshi Iwase, Shiang Jyi Hwang, Seina Ito, Kazuhisa Yamada, Shinji Ueno, Yasuki Ito, Hiroko Terasaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Purpose: Subsilicone oil fluid (SOF) in eyes with silicone oil (SO) endotamponade possibly has a role in complications (e.g., vision loss); thus, we aimed to examine inflammatory cytokine and electrolyte levels and retinal glial cell viability in SOF. Methods: We measured major inflammatory cytokine levels and electrolytes in SOF and compared them with those in vitreous fluid (VF) and anterior chamber fluid (ACF). We analyzed the correlation between inflammatory cytokines and retinal thickness in SO-filled eyes. Further, we measured the MIO-M1 cell viability in medium with SOF and compared it with that containing VF. Results: We collected and examined 57 SOF, 22 ACF, and 21 VF samples from eyes with PVR, PDR, RD, and MH. Interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 levels in SOF were significantly higher than those in ACF. There was no significant difference for all cytokines between SOF and VF. Retinal thickness changes during SO endotamponade were not correlated with the presence of any inflammatory cytokines. Levels of ferrous iron, but not of potassium, showed a significant decrease in SOF compared with VF. The WST-1 assay showed that SOF-added medium induced higher MIO-M1 cell viability than VF-added medium. Conclusions: We found no significant correlation between the change in the retinal thickness and cytokine levels, but SOF contains higher concentrations of cytokines and lower concentrations of ferrous iron and can be biologically distinguished from ACF and VF. Translational Relevance: Novel knowledge of inflammatory cytokine levels and electrolytes in SOF provides better understanding of pathology of SO-filled eyes.

Original languageEnglish
Article number28
JournalTranslational Vision Science and Technology
Volume8
Issue number1
DOIs
Publication statusPublished - 01-2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Ophthalmology

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