TY - JOUR
T1 - Biosynthesis of the so-called “a” and “asialo” pathway glycosphingolipids is differentially regulated in murine myelogenous leukemia nfs60 cells
AU - Nakamura, Mitsuru
AU - Sakai, Takao
AU - Furukawa, Yusuke
AU - Kitagawa, Seiichi
AU - Sakoe, Kumi
AU - Tsunoda, Atsuko
PY - 1995/12/26
Y1 - 1995/12/26
N2 - Regulation of “a” and “asialo” series ganglioside biosynthesis was analyzed. COS-1 cells expressing only GD1a showed high synthetic activities of GM3, GM2, GM1a, and GD1a, but little activity for GA2 synthesis. However, IL-3-dependent murine NFS60-I7, which has GM1b and GD1α, exhibited high synthetic activities of GM2, GM1a, and GD1a, but GM3 synthase was only 1/6 of COS-1 and GA2 synthetic activity was low. By contrast, IL-3 gene-transfected subline NFS60-H7 expressing GD1a in addition to GM1b and GD1α displayed up-regulated GM3 synthase and GA2 synthase activities, while GM2, GM1a, and GD1a synthase activities were in the same levels as in NFS60-I7 cells. Since GA2 synthetic activities were not parallel with GM2 synthase in the investigated machinery of ganglioside biosynthesis, it is strongly suggested that biosynthesis of and “asialo” series gangliosides is regulated differentially from each other in murine NFS60 cell lines.
AB - Regulation of “a” and “asialo” series ganglioside biosynthesis was analyzed. COS-1 cells expressing only GD1a showed high synthetic activities of GM3, GM2, GM1a, and GD1a, but little activity for GA2 synthesis. However, IL-3-dependent murine NFS60-I7, which has GM1b and GD1α, exhibited high synthetic activities of GM2, GM1a, and GD1a, but GM3 synthase was only 1/6 of COS-1 and GA2 synthetic activity was low. By contrast, IL-3 gene-transfected subline NFS60-H7 expressing GD1a in addition to GM1b and GD1α displayed up-regulated GM3 synthase and GA2 synthase activities, while GM2, GM1a, and GD1a synthase activities were in the same levels as in NFS60-I7 cells. Since GA2 synthetic activities were not parallel with GM2 synthase in the investigated machinery of ganglioside biosynthesis, it is strongly suggested that biosynthesis of and “asialo” series gangliosides is regulated differentially from each other in murine NFS60 cell lines.
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U2 - 10.1006/bbrc.1995.2834
DO - 10.1006/bbrc.1995.2834
M3 - Article
C2 - 8554592
AN - SCOPUS:0029417190
SN - 0006-291X
VL - 217
SP - 733
EP - 740
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -