There has been growing concern about the potential threat of hormone-disrupting chemicals like bisphenol A to various aspects of animal and human health. We studied the effects of bisphenol A on the Cl- secretion in human airway epithelial Calu-3 cells. Pretreatment with bisphenol A (IC50 = 60 μM, for 30 min) prevented isoproterenol (10 nM)-generated short-circuit current (Isc) more potently than 17 β-estradiol or tamoxifen (IC50 = 1 mM). 5′-Nitro-2-(3-phenylpropylamino) benzoate-sensitive apical conductance potentiated by isoproterenol was not affected by the pretreatment with either of these estrogenic compounds. The effects of bisphenol A were simulated in Isc responses to forskolin (10 μM) and 8-bromo-cAMP (1 mM). Nystatin permeabilization of Calu-3 monolayers revealed that bisphenol A attenuated 8-bromo-cAMP-induced basolateral K+ current, which is sensitive to clotrimazole (30 μM) and insensitive to charybdotoxin (100 nM), without affecting the apical CI- current. Bisphenol A, but neither 17 β-estradiol nor tamoxifen, interrupted the charybdotoxin-sensitive component of /sc stimulated by 1-ethyl-2-benzimidazolinone (1-EBIO; 500 μM). The inhibitory effects of bisphenol A on these Cl- secretory stimuli were remarkable when applied to the apical rather than the basolateral membrane. Alternatively, long-term incubation of bisphenol A (1 μM; 12-72 h) had no discernible effect on isoproterenol- and 1-EBIO-induced Cl- secretion. These findings indicate that short-term exposure to bisphenol A attenuates transepithelial CI- secretion through inhibition of both cAMP- and Ca2+-activated K+ channels on the basolateral membrane, interacting from the cytosolic surface in Calu-3 cells.
|Number of pages||8|
|Journal||Journal of Pharmacology and Experimental Therapeutics|
|Publication status||Published - 2002|
All Science Journal Classification (ASJC) codes
- Molecular Medicine