BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm

Hiroshi Ochiai, Hidetaka Suga, Tomiko Yamada, Mayu Sakakibara, Takatoshi Kasai, Chikafumi Ozone, Koichiro Ogawa, Motomitsu Goto, Ryoichi Banno, Shin Tsunekawa, Yoshihisa Sugimura, Hiroshi Arima, Yutaka Oiso

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

During embryonic development, oral ectoderm differentiates into the adenohypophysis, dental epithelia, salivary glands, and nasal pit. Few reports exist concerning the induction of oral ectoderm from embryonic stem (ES) cells. Generally, any lot differences in fetal bovine serum (FBS) and serum replacer may affect the induction of ES cell-differentiation. Using a previously established culture strategy for differentiation, the proportion of cell aggregates containing Pitx1. + oral ectoderm varied widely between 9-36% when several different lots of FBS or serum replacer were used. We therefore tried to enhance the differentiation method. We found that bone morphogenetic protein (BMP) 4 and fibroblast growth factor (FGF) treatments improved oral ectoderm induction. Such treatment also improved the differentiation of oral ectoderm into the adenohypophysis. Furthermore, increased BMP4 treatment induced dental epithelium and mesenchyme. Such differentiation suggests that the Pitx1. + layer displays similar properties to oral ectoderm, as found in vivo. Differentiation of ES cells into oral ectoderm using different lots of FBS and serum replacer increased 78-90% after treatment with BMP4 and FGF. In summary, we have established a robust strategy for the induction of oral ectoderm differentiation from mouse ES cells.

Original languageEnglish
Pages (from-to)290-298
Number of pages9
JournalStem Cell Research
Volume15
Issue number2
DOIs
Publication statusPublished - 01-09-2015

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Ectoderm
Fibroblast Growth Factors
Embryonic Stem Cells
Serum
Anterior Pituitary Gland
Cell Differentiation
Tooth
Epithelium
Bone Morphogenetic Protein 4
Mouse Embryonic Stem Cells
Mesoderm
Salivary Glands
Nose
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Ochiai, H., Suga, H., Yamada, T., Sakakibara, M., Kasai, T., Ozone, C., ... Oiso, Y. (2015). BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm. Stem Cell Research, 15(2), 290-298. https://doi.org/10.1016/j.scr.2015.06.011
Ochiai, Hiroshi ; Suga, Hidetaka ; Yamada, Tomiko ; Sakakibara, Mayu ; Kasai, Takatoshi ; Ozone, Chikafumi ; Ogawa, Koichiro ; Goto, Motomitsu ; Banno, Ryoichi ; Tsunekawa, Shin ; Sugimura, Yoshihisa ; Arima, Hiroshi ; Oiso, Yutaka. / BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm. In: Stem Cell Research. 2015 ; Vol. 15, No. 2. pp. 290-298.
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Ochiai, H, Suga, H, Yamada, T, Sakakibara, M, Kasai, T, Ozone, C, Ogawa, K, Goto, M, Banno, R, Tsunekawa, S, Sugimura, Y, Arima, H & Oiso, Y 2015, 'BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm', Stem Cell Research, vol. 15, no. 2, pp. 290-298. https://doi.org/10.1016/j.scr.2015.06.011

BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm. / Ochiai, Hiroshi; Suga, Hidetaka; Yamada, Tomiko; Sakakibara, Mayu; Kasai, Takatoshi; Ozone, Chikafumi; Ogawa, Koichiro; Goto, Motomitsu; Banno, Ryoichi; Tsunekawa, Shin; Sugimura, Yoshihisa; Arima, Hiroshi; Oiso, Yutaka.

In: Stem Cell Research, Vol. 15, No. 2, 01.09.2015, p. 290-298.

Research output: Contribution to journalArticle

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T1 - BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm

AU - Ochiai, Hiroshi

AU - Suga, Hidetaka

AU - Yamada, Tomiko

AU - Sakakibara, Mayu

AU - Kasai, Takatoshi

AU - Ozone, Chikafumi

AU - Ogawa, Koichiro

AU - Goto, Motomitsu

AU - Banno, Ryoichi

AU - Tsunekawa, Shin

AU - Sugimura, Yoshihisa

AU - Arima, Hiroshi

AU - Oiso, Yutaka

PY - 2015/9/1

Y1 - 2015/9/1

N2 - During embryonic development, oral ectoderm differentiates into the adenohypophysis, dental epithelia, salivary glands, and nasal pit. Few reports exist concerning the induction of oral ectoderm from embryonic stem (ES) cells. Generally, any lot differences in fetal bovine serum (FBS) and serum replacer may affect the induction of ES cell-differentiation. Using a previously established culture strategy for differentiation, the proportion of cell aggregates containing Pitx1. + oral ectoderm varied widely between 9-36% when several different lots of FBS or serum replacer were used. We therefore tried to enhance the differentiation method. We found that bone morphogenetic protein (BMP) 4 and fibroblast growth factor (FGF) treatments improved oral ectoderm induction. Such treatment also improved the differentiation of oral ectoderm into the adenohypophysis. Furthermore, increased BMP4 treatment induced dental epithelium and mesenchyme. Such differentiation suggests that the Pitx1. + layer displays similar properties to oral ectoderm, as found in vivo. Differentiation of ES cells into oral ectoderm using different lots of FBS and serum replacer increased 78-90% after treatment with BMP4 and FGF. In summary, we have established a robust strategy for the induction of oral ectoderm differentiation from mouse ES cells.

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Ochiai H, Suga H, Yamada T, Sakakibara M, Kasai T, Ozone C et al. BMP4 and FGF strongly induce differentiation of mouse ES cells into oral ectoderm. Stem Cell Research. 2015 Sep 1;15(2):290-298. https://doi.org/10.1016/j.scr.2015.06.011