TY - JOUR
T1 - Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins
AU - Hiraoka, Kunihiko
AU - Kaibuchi, Kozo
AU - Ando, Satoshi
AU - Musha, Takashi
AU - Takaishi, Kenji
AU - Mizuno, Takakazu
AU - Asada, Makoto
AU - Ménard, Luc
AU - Tomhave, Eric
AU - Didsbury, John
AU - Snyderman, Ralph
AU - Takai, Yoshimi
N1 - Funding Information:
' The investigation at Kobe University was supported by grants-in-aid for Scientific Research and for Cancer Research the Ministry of Education, Science, and Culture, Japan (1991), grants-in-aid for Abnormalities in Hormone Receptor Mechanisms for Aging and Health from the Ministry of Health and Welfare, (1991) I and grants from the Yamanouchi Foundation for Research Metabolic Disease (1991) and from the Research Program on Cell Calcium Signal in the Cardiovascular System (1991).
PY - 1992/1/31
Y1 - 1992/1/31
N2 - Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg rap1 p21, two peaks of rac1 p21, and one peak of an unidentified small G protein with a Mr of about 20,000 (20 KG). smg GDS, previously thought to be a stimulatory GDP GTP exchange protein for smg p21, Ki-ras p21, and rho p21, but not for Ha-ras p21 or smg p25A, was also active on rac1 p21. rho GDI, previously thought to be an inhibitory GDP GTP exchange protein specific for rho p21, was also active on rac1 p21. These results indicate that both smg GDS and rho GDI are active on multiple small G proteins.
AB - Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg rap1 p21, two peaks of rac1 p21, and one peak of an unidentified small G protein with a Mr of about 20,000 (20 KG). smg GDS, previously thought to be a stimulatory GDP GTP exchange protein for smg p21, Ki-ras p21, and rho p21, but not for Ha-ras p21 or smg p25A, was also active on rac1 p21. rho GDI, previously thought to be an inhibitory GDP GTP exchange protein specific for rho p21, was also active on rac1 p21. These results indicate that both smg GDS and rho GDI are active on multiple small G proteins.
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U2 - 10.1016/0006-291X(92)91820-G
DO - 10.1016/0006-291X(92)91820-G
M3 - Article
C2 - 1734890
AN - SCOPUS:0026501099
VL - 182
SP - 921
EP - 930
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -