Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins

Kunihiko Hiraoka; Kozo Kaibuchi; Satoshi Ando; Takashi Musha; Kenji Takaishi; Takakazu Mizuno; Makoto Asada; Luc Ménard; Eric Tomhave; John Didsbury; Ralph Snyderman; Yoshimi Takai

doi:10.1016/0006-291X(92)91820-G

Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins

Kunihiko Hiraoka, Kozo Kaibuchi, Satoshi Ando, Takashi Musha, Kenji Takaishi, Takakazu Mizuno, Makoto Asada, Luc Ménard, Eric Tomhave, John Didsbury, Ralph Snyderman, Yoshimi Takai

Institute for Comprehensive Medical Science

Research output: Contribution to journal › Article

80 Citations (Scopus)

Abstract

Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg rap1 p21, two peaks of rac1 p21, and one peak of an unidentified small G protein with a Mr of about 20,000 (20 KG). smg GDS, previously thought to be a stimulatory GDP GTP exchange protein for smg p21, Ki-ras p21, and rho p21, but not for Ha-ras p21 or smg p25A, was also active on rac1 p21. rho GDI, previously thought to be an inhibitory GDP GTP exchange protein specific for rho p21, was also active on rac1 p21. These results indicate that both smg GDS and rho GDI are active on multiple small G proteins.

Original language	English
Pages (from-to)	921-930
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91820-G
Publication status	Published - 31-01-1992

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Hiraoka, K., Kaibuchi, K., Ando, S., Musha, T., Takaishi, K., Mizuno, T., Asada, M., Ménard, L., Tomhave, E., Didsbury, J., Snyderman, R., & Takai, Y. (1992). Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins. Biochemical and Biophysical Research Communications, 182(2), 921-930. https://doi.org/10.1016/0006-291X(92)91820-G

Hiraoka, Kunihiko ; Kaibuchi, Kozo ; Ando, Satoshi ; Musha, Takashi ; Takaishi, Kenji ; Mizuno, Takakazu ; Asada, Makoto ; Ménard, Luc ; Tomhave, Eric ; Didsbury, John ; Snyderman, Ralph ; Takai, Yoshimi. / Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 182, No. 2. pp. 921-930.

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title = "Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins",

abstract = "Six peaks of small GTP-binding proteins (G proteins) were separated by column chromatographies from the cytosol fraction of the differentiated HL-60 cells: two peaks of rho p21, one peak of smg rap1 p21, two peaks of rac1 p21, and one peak of an unidentified small G protein with a Mr of about 20,000 (20 KG). smg GDS, previously thought to be a stimulatory GDP GTP exchange protein for smg p21, Ki-ras p21, and rho p21, but not for Ha-ras p21 or smg p25A, was also active on rac1 p21. rho GDI, previously thought to be an inhibitory GDP GTP exchange protein specific for rho p21, was also active on rac1 p21. These results indicate that both smg GDS and rho GDI are active on multiple small G proteins.",

author = "Kunihiko Hiraoka and Kozo Kaibuchi and Satoshi Ando and Takashi Musha and Kenji Takaishi and Takakazu Mizuno and Makoto Asada and Luc M{\'e}nard and Eric Tomhave and John Didsbury and Ralph Snyderman and Yoshimi Takai",

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doi = "10.1016/0006-291X(92)91820-G",

language = "English",

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Hiraoka, K, Kaibuchi, K, Ando, S, Musha, T, Takaishi, K, Mizuno, T, Asada, M, Ménard, L, Tomhave, E, Didsbury, J, Snyderman, R & Takai, Y 1992, 'Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins', Biochemical and Biophysical Research Communications, vol. 182, no. 2, pp. 921-930. https://doi.org/10.1016/0006-291X(92)91820-G

Both stimulatory and inhibitory GDP GTP exchange proteins, smg GDS and rho GDI, are active on multiple small GTP-binding proteins. / Hiraoka, Kunihiko; Kaibuchi, Kozo; Ando, Satoshi; Musha, Takashi; Takaishi, Kenji; Mizuno, Takakazu; Asada, Makoto; Ménard, Luc; Tomhave, Eric; Didsbury, John; Snyderman, Ralph; Takai, Yoshimi.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 2, 31.01.1992, p. 921-930.

Research output: Contribution to journal › Article

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AU - Hiraoka, Kunihiko

AU - Kaibuchi, Kozo

AU - Ando, Satoshi

AU - Musha, Takashi

AU - Takaishi, Kenji

AU - Mizuno, Takakazu

AU - Asada, Makoto

AU - Ménard, Luc

AU - Tomhave, Eric

AU - Didsbury, John

AU - Snyderman, Ralph

AU - Takai, Yoshimi

PY - 1992/1/31

Y1 - 1992/1/31

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