Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus

Akiko Kondo, Keisuke Hitachi, Tempei Ikegame, Makoto Asashima

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cells in the prospective somite of Xenopus laevis embryos rotate in an orchestrated manner to form a segregated somite. The prospective somite boundaries are prepatterned by gene expressions in the unsegmented presomitic mesoderm (PSM). However, the roles of polarized gene expression in this boundary formation are not well elucidated. Here we identified a novel gene, bowline, which localizes to the anterior halves of S-II, III in the PSM of X. laevis. Bowline associated with corepressor XGrg-4, a Xenopus homolog of Groucho/TLE protein. A WRPW tetrapeptide motif in Bowline was prerequisite for coprecipitation with XGrg-4 and for downregulation of X-Delta-2 by bowline RNA injection. This study indicates that Bowline is a novel protein interacting with Groucho/TLE and may play a role in somitogenesis in X. laevis.

Original languageEnglish
Pages (from-to)473-479
Number of pages7
JournalInternational Journal of Developmental Biology
Volume50
Issue number5
DOIs
Publication statusPublished - 14-06-2006

Fingerprint

Somites
Xenopus laevis
Mesoderm
Xenopus
Gene Expression
Co-Repressor Proteins
Proteins
Down-Regulation
Embryonic Structures
RNA
Injections
Genes

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

@article{f02bcfdbe4b24c059a71a41d7eb6dd35,
title = "Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus",
abstract = "Cells in the prospective somite of Xenopus laevis embryos rotate in an orchestrated manner to form a segregated somite. The prospective somite boundaries are prepatterned by gene expressions in the unsegmented presomitic mesoderm (PSM). However, the roles of polarized gene expression in this boundary formation are not well elucidated. Here we identified a novel gene, bowline, which localizes to the anterior halves of S-II, III in the PSM of X. laevis. Bowline associated with corepressor XGrg-4, a Xenopus homolog of Groucho/TLE protein. A WRPW tetrapeptide motif in Bowline was prerequisite for coprecipitation with XGrg-4 and for downregulation of X-Delta-2 by bowline RNA injection. This study indicates that Bowline is a novel protein interacting with Groucho/TLE and may play a role in somitogenesis in X. laevis.",
author = "Akiko Kondo and Keisuke Hitachi and Tempei Ikegame and Makoto Asashima",
year = "2006",
month = "6",
day = "14",
doi = "10.1387/ijdb.052138ak",
language = "English",
volume = "50",
pages = "473--479",
journal = "International Journal of Developmental Biology",
issn = "0214-6282",
publisher = "University of the Basque Country Press",
number = "5",

}

Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus. / Kondo, Akiko; Hitachi, Keisuke; Ikegame, Tempei; Asashima, Makoto.

In: International Journal of Developmental Biology, Vol. 50, No. 5, 14.06.2006, p. 473-479.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bowline, a novel protein localized to the presomitic mesoderm, interacts with Groucho/TLE in Xenopus

AU - Kondo, Akiko

AU - Hitachi, Keisuke

AU - Ikegame, Tempei

AU - Asashima, Makoto

PY - 2006/6/14

Y1 - 2006/6/14

N2 - Cells in the prospective somite of Xenopus laevis embryos rotate in an orchestrated manner to form a segregated somite. The prospective somite boundaries are prepatterned by gene expressions in the unsegmented presomitic mesoderm (PSM). However, the roles of polarized gene expression in this boundary formation are not well elucidated. Here we identified a novel gene, bowline, which localizes to the anterior halves of S-II, III in the PSM of X. laevis. Bowline associated with corepressor XGrg-4, a Xenopus homolog of Groucho/TLE protein. A WRPW tetrapeptide motif in Bowline was prerequisite for coprecipitation with XGrg-4 and for downregulation of X-Delta-2 by bowline RNA injection. This study indicates that Bowline is a novel protein interacting with Groucho/TLE and may play a role in somitogenesis in X. laevis.

AB - Cells in the prospective somite of Xenopus laevis embryos rotate in an orchestrated manner to form a segregated somite. The prospective somite boundaries are prepatterned by gene expressions in the unsegmented presomitic mesoderm (PSM). However, the roles of polarized gene expression in this boundary formation are not well elucidated. Here we identified a novel gene, bowline, which localizes to the anterior halves of S-II, III in the PSM of X. laevis. Bowline associated with corepressor XGrg-4, a Xenopus homolog of Groucho/TLE protein. A WRPW tetrapeptide motif in Bowline was prerequisite for coprecipitation with XGrg-4 and for downregulation of X-Delta-2 by bowline RNA injection. This study indicates that Bowline is a novel protein interacting with Groucho/TLE and may play a role in somitogenesis in X. laevis.

UR - http://www.scopus.com/inward/record.url?scp=33744908068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744908068&partnerID=8YFLogxK

U2 - 10.1387/ijdb.052138ak

DO - 10.1387/ijdb.052138ak

M3 - Article

C2 - 16586348

AN - SCOPUS:33744908068

VL - 50

SP - 473

EP - 479

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 5

ER -