Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development

Shingo Suzuki, Kazuyuki Kiyosue, Shunsuke Hazama, Akihiko Ogura, Megumi Kashihara, Tomoko Hara, Hisatsugu Koshimizu, Masami Kojima

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Brain-derived neurotrophic factor (BDNF) exerts multiple biological functions in the CNS. Although BDNF can control transcription and protein synthesis, it still remains open to question whether BDNF regulates lipid biosynthesis. Here we show that BDNF elicits cholesterol biosynthesis in cultured cortical and hippocampal neurons. Importantly, BDNF elicited cholesterol synthesis in neurons, but not in glial cells. Quantitative reverse transcriptase-PCR revealed that BDNF stimulated the transcription of enzymes in the cholesterol biosynthetic pathway. BDNF-induced cholesterol increases were blocked by specific inhibitors of cholesterol synthesis, mevastatin and zaragozic acid, suggesting that BDNF stimulates de novo synthesis of cholesterol rather than the incorporation of extracellular cholesterol. Because cholesterol is a major component of lipid rafts, we investigated whether BDNF would increase the cholesterol content in lipid rafts or nonraft membrane domains. Interestingly, the BDNF-mediated increase in cholesterol occurred in rafts, but not in nonrafts, suggesting that BDNF promotes the development of neuronal lipid rafts. Consistent with this notion, BDNF raised the level of the lipid raft marker protein caveolin-2 in rafts. Remarkably, BDNF increased the levels of presynaptic proteins in lipid rafts, but not in nonrafts. An electrophysiological study revealed that BDNF-dependent cholesterol biosynthesis plays an important role for the development of a readily releasable pool of synaptic vesicles. Together, these results suggest a novel role for BDNF in cholesterol metabolism and synapse development.

Original languageEnglish
Pages (from-to)6417-6427
Number of pages11
JournalJournal of Neuroscience
Volume27
Issue number24
DOIs
Publication statusPublished - 13-06-2007

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Brain-Derived Neurotrophic Factor
Synapses
Cholesterol
Lipids
Caveolin 2
Anticholesteremic Agents
Neurons
Proteins
Synaptic Vesicles
Biosynthetic Pathways
Reverse Transcriptase Polymerase Chain Reaction
Neuroglia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Suzuki, S., Kiyosue, K., Hazama, S., Ogura, A., Kashihara, M., Hara, T., ... Kojima, M. (2007). Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development. Journal of Neuroscience, 27(24), 6417-6427. https://doi.org/10.1523/JNEUROSCI.0690-07.2007
Suzuki, Shingo ; Kiyosue, Kazuyuki ; Hazama, Shunsuke ; Ogura, Akihiko ; Kashihara, Megumi ; Hara, Tomoko ; Koshimizu, Hisatsugu ; Kojima, Masami. / Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development. In: Journal of Neuroscience. 2007 ; Vol. 27, No. 24. pp. 6417-6427.
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Suzuki, S, Kiyosue, K, Hazama, S, Ogura, A, Kashihara, M, Hara, T, Koshimizu, H & Kojima, M 2007, 'Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development', Journal of Neuroscience, vol. 27, no. 24, pp. 6417-6427. https://doi.org/10.1523/JNEUROSCI.0690-07.2007

Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development. / Suzuki, Shingo; Kiyosue, Kazuyuki; Hazama, Shunsuke; Ogura, Akihiko; Kashihara, Megumi; Hara, Tomoko; Koshimizu, Hisatsugu; Kojima, Masami.

In: Journal of Neuroscience, Vol. 27, No. 24, 13.06.2007, p. 6417-6427.

Research output: Contribution to journalArticle

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T1 - Brain-derived neurotrophic factor regulates cholesterol metabolism for synapse development

AU - Suzuki, Shingo

AU - Kiyosue, Kazuyuki

AU - Hazama, Shunsuke

AU - Ogura, Akihiko

AU - Kashihara, Megumi

AU - Hara, Tomoko

AU - Koshimizu, Hisatsugu

AU - Kojima, Masami

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