TY - JOUR
T1 - Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain
AU - Sawada, Makoto
AU - Imai, Fumihiro
AU - Suzuki, Hiromi
AU - Hayakawa, Motoharu
AU - Kanno, Tetsuo
AU - Nagatsu, Toshiharu
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture, from Funds for Comprehensive Research on Aging and Health, and from Fujita Health University.
PY - 1998/8/14
Y1 - 1998/8/14
N2 - The intra-arterial injection of immortalized microglia transfected with the lacZ gene, resulted in the expression of β-galactosidase in the rat brain at 48 h and the activity of β-galactosidase was detected for up to 3 weeks post-injection. More than 30-fold higher activity of β-galactosidase was detected in the brain than in the liver, lung or spleen at 48 h post-injection. This method allows us to easily deliver the gene of interest to the brain without influencing other organs. Our brain-targeting gene delivery system can facilitate gene therapy of several brain disorders, including brain tumor, metabolic disorders, and degenerative disorders, as well as investigation into the roles of particular genes in brain function and development. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - The intra-arterial injection of immortalized microglia transfected with the lacZ gene, resulted in the expression of β-galactosidase in the rat brain at 48 h and the activity of β-galactosidase was detected for up to 3 weeks post-injection. More than 30-fold higher activity of β-galactosidase was detected in the brain than in the liver, lung or spleen at 48 h post-injection. This method allows us to easily deliver the gene of interest to the brain without influencing other organs. Our brain-targeting gene delivery system can facilitate gene therapy of several brain disorders, including brain tumor, metabolic disorders, and degenerative disorders, as well as investigation into the roles of particular genes in brain function and development. Copyright (C) 1998 Federation of European Biochemical Societies.
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U2 - 10.1016/S0014-5793(98)00879-5
DO - 10.1016/S0014-5793(98)00879-5
M3 - Article
C2 - 9738928
AN - SCOPUS:0031840591
SN - 0014-5793
VL - 433
SP - 37
EP - 40
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -