Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi; Koji Ohashi; I. D.E. Tomihiko; Jaime Pereda

doi:10.2535/ofaj.86.73

Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi, Koji Ohashi, I. D.E. Tomihiko, Jaime Pereda

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Abstract

This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

Original language	English
Pages (from-to)	73-77
Number of pages	5
Journal	Okajimas Folia Anatomica Japonica
Volume	86
Issue number	3
DOIs	https://doi.org/10.2535/ofaj.86.73
Publication status	Published - 11-2009

All Science Journal Classification (ASJC) codes

Anatomy

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10.2535/ofaj.86.73

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abstract = "This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.",

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AB - This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

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Budding and direct division of adult human erythrocytes in serum-free cultures

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