Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi, Koji Ohashi, I. D.E. Tomihiko, Jaime Pereda

Research output: Contribution to journalArticlepeer-review


This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalOkajimas Folia Anatomica Japonica
Issue number3
Publication statusPublished - 11-2009

All Science Journal Classification (ASJC) codes

  • Anatomy


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