Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi, Koji Ohashi, I. D.E. Tomihiko, Jaime Pereda

Research output: Contribution to journalArticle

Abstract

This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalOkajimas Folia Anatomica Japonica
Volume86
Issue number3
DOIs
Publication statusPublished - 01-11-2009

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Erythrocytes
Serum
Eagles
Erythropoiesis
Erythropoietin
Microscopy
Light
Food

All Science Journal Classification (ASJC) codes

  • Anatomy

Cite this

Niimi, Gen ; Ohashi, Koji ; Tomihiko, I. D.E. ; Pereda, Jaime. / Budding and direct division of adult human erythrocytes in serum-free cultures. In: Okajimas Folia Anatomica Japonica. 2009 ; Vol. 86, No. 3. pp. 73-77.
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Budding and direct division of adult human erythrocytes in serum-free cultures. / Niimi, Gen; Ohashi, Koji; Tomihiko, I. D.E.; Pereda, Jaime.

In: Okajimas Folia Anatomica Japonica, Vol. 86, No. 3, 01.11.2009, p. 73-77.

Research output: Contribution to journalArticle

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