Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi; Koji Ohashi; I. D.E. Tomihiko; Jaime Pereda

doi:10.2535/ofaj.86.73

Budding and direct division of adult human erythrocytes in serum-free cultures

Gen Niimi
, Koji Ohashi
, I. D.E. Tomihiko
, Jaime Pereda

Research output: Contribution to journal › Article › peer-review

Abstract

This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

Original language	English
Pages (from-to)	73-77
Number of pages	5
Journal	Okajimas Folia Anatomica Japonica
Volume	86
Issue number	3
DOIs	https://doi.org/10.2535/ofaj.86.73
Publication status	Published - 11-2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Anatomy

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10.2535/ofaj.86.73

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title = "Budding and direct division of adult human erythrocytes in serum-free cultures",

abstract = "This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.",

keywords = "Closed culture, Definitive erythropoiesis, Human, Peripheral blood, Red blood cell",

author = "Gen Niimi and Koji Ohashi and Tomihiko, \{I. D.E.\} and Jaime Pereda",

year = "2009",

month = nov,

doi = "10.2535/ofaj.86.73",

language = "English",

volume = "86",

pages = "73--77",

journal = "Okajimas Folia Anatomica Japonica",

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T1 - Budding and direct division of adult human erythrocytes in serum-free cultures

AU - Niimi, Gen

AU - Ohashi, Koji

AU - Tomihiko, I. D.E.

AU - Pereda, Jaime

PY - 2009/11

Y1 - 2009/11

N2 - This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

AB - This paper documents the budding and division process of human erythrocytes in vitro using time-lapse light microscopy of hanging-drop preparations. The erythrocytes were prepared from normal adult human peripheral blood. Red blood cells showed cytoplasmic budding, segmentation, and direct division with delayed addition of erythropoietin in serum-free Dulbecco's modified Eagle's medium/nutrient mixture F-12 Ham. These observations are thought to be useful for developing model of definitive erythropoiesis and simple expansion of human erythrocytes.

KW - Closed culture

KW - Definitive erythropoiesis

KW - Human

KW - Peripheral blood

KW - Red blood cell

UR - https://www.scopus.com/pages/publications/77949314283

UR - https://www.scopus.com/pages/publications/77949314283#tab=citedBy

U2 - 10.2535/ofaj.86.73

DO - 10.2535/ofaj.86.73

M3 - Article

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AN - SCOPUS:77949314283

SN - 0030-154X

VL - 86

SP - 73

EP - 77

JO - Okajimas Folia Anatomica Japonica

JF - Okajimas Folia Anatomica Japonica

IS - 3

ER -

Budding and direct division of adult human erythrocytes in serum-free cultures

Abstract

All Science Journal Classification (ASJC) codes

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