Butyrate enhances the production of nitric oxide in mouse vascular endothelial cells in response to gamma interferon

Akiko Morikawa, Tsuyoshi Sugiyama, Naoki Koide, Isamu Mori, Mya Mya Mu, Tomoaki Yoshida, Ferdaus Hassan, Shamima Islam, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The effect of butyrate, a natural bacterial product of colonic bacterial flora, on nitric oxide (NO) production in murine vascular endothelial cell line END-D in response to IFN-γ and/or LPS was studied. Butyrate significantly augmented NO production in END-D cells in response to IFN-γ or IFN-γ + LPS, but not LPS alone. The NO production was augmented by the addition of butyrate until 6 h after the stimulation with IFN-γ or IFN-γ + LPS. The augmentation was abolished by the removal of butyrate from the cultures. Butyrate enhanced the expression of inducible type NO synthase (iNOS) in the stimulated END-D cells. Furthermore, butyrate-enhanced NO production in the presence of various signal inhibitors down-regulating the signal pathways using nuclear factor (NF)-κB, mitogen-activated protein (MAP) kinases and Janus tyrosine kinase. The putative mechanism of butyrate-induced augmentation of NO production in response to IFN-γ or IFN-γ + LPS is discussed.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalJournal of Endotoxin Research
Volume10
Issue number1
DOIs
Publication statusPublished - 2004

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

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