Cancer-specifically re-spliced TSG101 mRNA promotes invasion and metastasis of nasopharyngeal carcinoma

Huey Huey Chua, Toshiki Kameyama, Akila Mayeda, Te Huei Yeh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

TSG101 (Tumor susceptibility 101) gene and its aberrantly spliced isoform, termed TSG101∆154-1054, are tightly linked to tumorigenesis in various cancers. The aberrant TSG101∆154-1054 mRNA is generated from cancer-specific re-splicing of mature TSG101 mRNA. The TSG101∆154-1054 protein protects the full-length TSG101 protein from ubiquitin-mediated degradation, implicating TSG101∆154-1054 protein in the progression of cancer. Here, we confirmed that the presence of TSG101∆154-1054 mRNA indeed caused an accumulation of the TSG101 protein in biopsies of human nasopharyngeal carcinoma (NPC), which was recapitulated by the overexpression of TSG101∆154-1054 in the NPC cell line TW01. We demonstrate the potential function of the TSG101∆154-1054 protein in the malignancy of human NPC with scratch-wound healing and transwell invasion assays. By increasing the stability of the TSG101 protein, TSG101∆154-1054 specifically enhanced TSG101-mediated TW01 cell migration and invasion, suggesting the involvement in NPC metastasis in vivo. This finding sheds light on the functional significance of TSG101∆154-1054 generation via re-splicing of TSG101 mRNA in NPC metastasis and hints at its potential importance as a therapeutic target.

Original languageEnglish
Article number773
JournalInternational journal of molecular sciences
Volume20
Issue number3
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

metastasis
cancer
Neoplasm Metastasis
Proteins
Messenger RNA
proteins
Neoplasms
splicing
Biopsy
Ubiquitin
Assays
Protein Isoforms
Wound Healing
Cells
Cell Movement
Carcinogenesis
wound healing
Degradation
Nasopharyngeal carcinoma
Tsg101 protein

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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title = "Cancer-specifically re-spliced TSG101 mRNA promotes invasion and metastasis of nasopharyngeal carcinoma",
abstract = "TSG101 (Tumor susceptibility 101) gene and its aberrantly spliced isoform, termed TSG101∆154-1054, are tightly linked to tumorigenesis in various cancers. The aberrant TSG101∆154-1054 mRNA is generated from cancer-specific re-splicing of mature TSG101 mRNA. The TSG101∆154-1054 protein protects the full-length TSG101 protein from ubiquitin-mediated degradation, implicating TSG101∆154-1054 protein in the progression of cancer. Here, we confirmed that the presence of TSG101∆154-1054 mRNA indeed caused an accumulation of the TSG101 protein in biopsies of human nasopharyngeal carcinoma (NPC), which was recapitulated by the overexpression of TSG101∆154-1054 in the NPC cell line TW01. We demonstrate the potential function of the TSG101∆154-1054 protein in the malignancy of human NPC with scratch-wound healing and transwell invasion assays. By increasing the stability of the TSG101 protein, TSG101∆154-1054 specifically enhanced TSG101-mediated TW01 cell migration and invasion, suggesting the involvement in NPC metastasis in vivo. This finding sheds light on the functional significance of TSG101∆154-1054 generation via re-splicing of TSG101 mRNA in NPC metastasis and hints at its potential importance as a therapeutic target.",
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Cancer-specifically re-spliced TSG101 mRNA promotes invasion and metastasis of nasopharyngeal carcinoma. / Chua, Huey Huey; Kameyama, Toshiki; Mayeda, Akila; Yeh, Te Huei.

In: International journal of molecular sciences, Vol. 20, No. 3, 773, 01.01.2019.

Research output: Contribution to journalArticle

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