Abstract
We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
| Original language | English |
|---|---|
| Pages (from-to) | 10055-10060 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 81 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 15-12-2009 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose. / Dang, Fuquan; Maeda, Eiki; Osafune, Tomo; Nakajima, Kazuki; Kakehi, Kazuaki; Ishikawa, Mitsuru; Baba, Yoshinobu.
In: Analytical Chemistry, Vol. 81, No. 24, 15.12.2009, p. 10055-10060.Research output: Contribution to journal › Article
TY - JOUR
T1 - Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose
AU - Dang, Fuquan
AU - Maeda, Eiki
AU - Osafune, Tomo
AU - Nakajima, Kazuki
AU - Kakehi, Kazuaki
AU - Ishikawa, Mitsuru
AU - Baba, Yoshinobu
PY - 2009/12/15
Y1 - 2009/12/15
N2 - We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
AB - We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
UR - http://www.scopus.com/inward/record.url?scp=72449139501&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72449139501&partnerID=8YFLogxK
U2 - 10.1021/ac902014c
DO - 10.1021/ac902014c
M3 - Article
C2 - 19902929
AN - SCOPUS:72449139501
VL - 81
SP - 10055
EP - 10060
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 24
ER -