Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Fuquan Dang, Eiki Maeda, Tomo Osafune, Kazuki Nakajima, Kazuaki Kakehi, Mitsuru Ishikawa, Yoshinobu Baba

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

Original languageEnglish
Pages (from-to)10055-10060
Number of pages6
JournalAnalytical Chemistry
Volume81
Issue number24
DOIs
Publication statusPublished - 15-12-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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