Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Fuquan Dang, Eiki Maeda, Tomo Osafune, Kazuki Nakajima, Kazuaki Kakehi, Mitsuru Ishikawa, Yoshinobu Baba

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

Original languageEnglish
Pages (from-to)10055-10060
Number of pages6
JournalAnalytical Chemistry
Volume81
Issue number24
DOIs
Publication statusPublished - 15-12-2009

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Polymethyl Methacrylate
Carbohydrates
Plastics
Self assembled monolayers
Proteins
Molecular interactions
Complex Mixtures
Polysaccharides
Glycoproteins
Screening
Phosphates
Coatings
Acids
hydroxyethylcellulose
Water

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Dang, Fuquan ; Maeda, Eiki ; Osafune, Tomo ; Nakajima, Kazuki ; Kakehi, Kazuaki ; Ishikawa, Mitsuru ; Baba, Yoshinobu. / Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose. In: Analytical Chemistry. 2009 ; Vol. 81, No. 24. pp. 10055-10060.
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Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose. / Dang, Fuquan; Maeda, Eiki; Osafune, Tomo; Nakajima, Kazuki; Kakehi, Kazuaki; Ishikawa, Mitsuru; Baba, Yoshinobu.

In: Analytical Chemistry, Vol. 81, No. 24, 15.12.2009, p. 10055-10060.

Research output: Contribution to journalArticle

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AU - Dang, Fuquan

AU - Maeda, Eiki

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AU - Baba, Yoshinobu

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