Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Fuquan Dang; Eiki Maeda; Tomo Osafune; Kazuki Nakajima; Kazuaki Kakehi; Mitsuru Ishikawa; Yoshinobu Baba

doi:10.1021/ac902014c

Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Fuquan Dang, Eiki Maeda, Tomo Osafune, Kazuki Nakajima, Kazuaki Kakehi, Mitsuru Ishikawa, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

Original language	English
Pages (from-to)	10055-10060
Number of pages	6
Journal	Analytical Chemistry
Volume	81
Issue number	24
DOIs	https://doi.org/10.1021/ac902014c
Publication status	Published - 15-12-2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Access to Document

10.1021/ac902014c

Cite this

@article{891bc268ea0b41b5b04ed7e4f025d2fc,

title = "Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose",

abstract = "We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.",

author = "Fuquan Dang and Eiki Maeda and Tomo Osafune and Kazuki Nakajima and Kazuaki Kakehi and Mitsuru Ishikawa and Yoshinobu Baba",

year = "2009",

month = dec,

day = "15",

doi = "10.1021/ac902014c",

language = "English",

volume = "81",

pages = "10055--10060",

journal = "Analytical Chemistry",

issn = "0003-2700",

number = "24",

}

TY - JOUR

T1 - Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

AU - Dang, Fuquan

AU - Maeda, Eiki

AU - Osafune, Tomo

AU - Nakajima, Kazuki

AU - Kakehi, Kazuaki

AU - Ishikawa, Mitsuru

AU - Baba, Yoshinobu

PY - 2009/12/15

Y1 - 2009/12/15

N2 - We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

AB - We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.

UR - http://www.scopus.com/inward/record.url?scp=72449139501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72449139501&partnerID=8YFLogxK

U2 - 10.1021/ac902014c

DO - 10.1021/ac902014c

M3 - Article

C2 - 19902929

AN - SCOPUS:72449139501

VL - 81

SP - 10055

EP - 10060

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 24

ER -

Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified hydroxyethylcellulose

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this