TY - JOUR
T1 - Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum
AU - Yoshida, Motonobu
AU - Matsui, Taei
AU - Fuse, Goro
AU - Ouchi, Seiji
N1 - Funding Information:
Acknowledgements:W e thankD rs. Ten Feizi and Akira Hayashi for critically reading the manuscript. This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan and a grant from Kinki University.
PY - 1993/3/8
Y1 - 1993/3/8
N2 - We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.
AB - We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.
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U2 - 10.1016/0014-5793(93)80534-2
DO - 10.1016/0014-5793(93)80534-2
M3 - Article
C2 - 8440387
AN - SCOPUS:0027512914
SN - 0014-5793
VL - 318
SP - 305
EP - 309
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -