Carbohydrate structures of the cell adhesion molecule, contact site A, from Dictyostelium discoideum

Motonobu Yoshida, Taei Matsui, Goro Fuse, Seiji Ouchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We determined the carbohydrate structures of contact site A from Dictyostelium discoideum. The carbohydrate moieties of contact site A were released by hydrazinolysis. Fractionation of the deacidified oligosaccharide mixture by Bio-Gel P-4 column chromatography revealed that it was composed of four major oligosaccharides. Their respective structures were determined by sequential exoglycosidase digestion. It is known that contact site A consists of two kinds of carbohydrates, I and II. Taking together the previous and the present results, it was deduced that carbohydrate I comprises N-linked oligosaccharides and carbohydrate II O-linked ones. Furthermore, the relative molar contents of GalNAc and GlcNAc in reducing terminal suggested that contact site A contains 67% of N-linked and 33% of O-linked oligosaccharides.

Original languageEnglish
Pages (from-to)305-309
Number of pages5
JournalFEBS Letters
Volume318
Issue number3
DOIs
Publication statusPublished - 08-03-1993

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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