Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model

Hanayuki Okura, Akifumi Matsuyama, Chun Man Lee, Ayami Saga, Aya Kakuta-Yamamoto, Anna Nagao, Nagako Sougawa, Naosumi Sekiya, Kazuhiro Takekita, Yashuhiro Shudo, Shigeru Miyagawa, Hiroshi Komoda, Teruo Okano, Yoshiki Sawa

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Adipose tissue-derived mesenchymal stem cells (ADMSCs) are multipotent cells. Here we examined whether human ADMSCs (hADMSCs) could differentiate into cardiomyoblast-like cells (CLCs) by induction with dimethylsulfoxide and whether the cells would be utilized to treat cardiac dysfunction. Dimethylsulfoxide induced the expression of various cardiac markers in hADMSCs, such as α-cardiac actin, cardiac myosin light chain, and myosin heavy chain; none of which were detected in noncommitted hADMSCs. The induced cells were thus designated as hADMSC-derived CLCs (hCLCs). To confirm their beneficial effect on cardiac function, hCLC patches were transplanted onto the Nude rat myocardial infarction model, and compared with noncommitted hADMSC patch transplants and sham operations. Echocardiography demonstrated significant short-term improvement of cardiac function in both the patch-transplanted groups. However, long-term follow-up showed rescue and maintenance of cardiac function in the hCLC patch-transplanted group only, but not in the noncommitted hADMSC patch-transplanted animals. The hCLCs, but not the hADMSCs, engrafted into the scarred myocardium and differentiated into human cardiac troponin I-positive cells, and thus regarded as cardiomyocytes. Transplantation of the hCLC patches also resulted in recovery of cardiac function and improvement of long-term survival rate. Thus, transplantation of hCLC patches is a potentially effective therapeutic strategy for future cardiac tissue regeneration.

Original languageEnglish
Pages (from-to)417-425
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume16
Issue number3
DOIs
Publication statusPublished - 01-06-2010

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Left Ventricular Dysfunction
Stem cells
Mesenchymal Stromal Cells
Adipose Tissue
Rats
Myocardial Infarction
Tissue
Survival
Dimethyl Sulfoxide
Cardiac Myosins
Echocardiography
Tissue regeneration
Myosin Light Chains
Transplants
Troponin I
Myosin Heavy Chains
Actins
Animals
Transplantation
Cells

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Okura, Hanayuki ; Matsuyama, Akifumi ; Lee, Chun Man ; Saga, Ayami ; Kakuta-Yamamoto, Aya ; Nagao, Anna ; Sougawa, Nagako ; Sekiya, Naosumi ; Takekita, Kazuhiro ; Shudo, Yashuhiro ; Miyagawa, Shigeru ; Komoda, Hiroshi ; Okano, Teruo ; Sawa, Yoshiki. / Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model. In: Tissue Engineering - Part C: Methods. 2010 ; Vol. 16, No. 3. pp. 417-425.
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Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model. / Okura, Hanayuki; Matsuyama, Akifumi; Lee, Chun Man; Saga, Ayami; Kakuta-Yamamoto, Aya; Nagao, Anna; Sougawa, Nagako; Sekiya, Naosumi; Takekita, Kazuhiro; Shudo, Yashuhiro; Miyagawa, Shigeru; Komoda, Hiroshi; Okano, Teruo; Sawa, Yoshiki.

In: Tissue Engineering - Part C: Methods, Vol. 16, No. 3, 01.06.2010, p. 417-425.

Research output: Contribution to journalArticle

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T1 - Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model

AU - Okura, Hanayuki

AU - Matsuyama, Akifumi

AU - Lee, Chun Man

AU - Saga, Ayami

AU - Kakuta-Yamamoto, Aya

AU - Nagao, Anna

AU - Sougawa, Nagako

AU - Sekiya, Naosumi

AU - Takekita, Kazuhiro

AU - Shudo, Yashuhiro

AU - Miyagawa, Shigeru

AU - Komoda, Hiroshi

AU - Okano, Teruo

AU - Sawa, Yoshiki

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