Cardiomyocyte transplantation in a porcine myocardial infarction model

Eiichi Watanabe, Duane M. Smith, Joseph B. Delcarpio, Jian Sun, Frank W. Smart, Clifford H. Van Meter, William C. Claycomb

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Transplantation of cardiomyocytes into the heart is a potential treatment for replacing damaged cardiac muscle. To investigate the feasibility and efficiency of this technique, either a cardiac-derived cell line (HL-1 cells), or normal fetal or neonatal pig cardiomyocytes were grafted into a porcine model of myocardial infarction. The myocardial infarction was created by the placement of an embolization coil in the distal portion of the left anterior descending artery in Yorkshire pigs (n = 9). Four to 5 wk after creation of an infarct, the three preparations of cardiomyocytes were grafted, at 1 x 106 cells/20 μL into normal and into the middle of the infarcted myocardium. The hearts were harvested and processed for histologic examinations 4 to 5 wk after the cell grafts. Histologic evaluation of the graft sites demonstrated that HL-1 cells and fetal pig cardiomyocytes formed stable grafts within the normal myocardium without any detrimental effect including arrhythmia. In addition, a marked increase in angiogenesis was observed both within the grafts and adjacent host myocardium. Electron microscopy studies demonstrated that fetal pig cardiomyocytes and the host myocardial cells were coupled with adherens-type junctions and gap junctions. Histologic examination of graft sites from infarct tissue failed to show the presence of grafted HL-1 cells, fetal, or neonatal pig cardiomyocytes. Cardiomyocyte transplantation may provide the potential means for cell-mediated gene therapy for introduction of therapeutic molecules into the heart.

Original languageEnglish
Pages (from-to)239-246
Number of pages8
JournalCell Transplantation
Volume7
Issue number3
DOIs
Publication statusPublished - 01-05-1998

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Cardiac Myocytes
Grafts
Swine
Transplantation
Myocardial Infarction
Myocardium
Transplants
Gene therapy
Adherens Junctions
Electron microscopy
Muscle
Gap Junctions
Cells
Tissue
Genetic Therapy
Cardiac Arrhythmias
Molecules
Electron Microscopy
Arteries
Cell Line

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Watanabe, E., Smith, D. M., Delcarpio, J. B., Sun, J., Smart, F. W., Van Meter, C. H., & Claycomb, W. C. (1998). Cardiomyocyte transplantation in a porcine myocardial infarction model. Cell Transplantation, 7(3), 239-246. https://doi.org/10.1016/S0963-6897(98)00011-6
Watanabe, Eiichi ; Smith, Duane M. ; Delcarpio, Joseph B. ; Sun, Jian ; Smart, Frank W. ; Van Meter, Clifford H. ; Claycomb, William C. / Cardiomyocyte transplantation in a porcine myocardial infarction model. In: Cell Transplantation. 1998 ; Vol. 7, No. 3. pp. 239-246.
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Watanabe, E, Smith, DM, Delcarpio, JB, Sun, J, Smart, FW, Van Meter, CH & Claycomb, WC 1998, 'Cardiomyocyte transplantation in a porcine myocardial infarction model', Cell Transplantation, vol. 7, no. 3, pp. 239-246. https://doi.org/10.1016/S0963-6897(98)00011-6

Cardiomyocyte transplantation in a porcine myocardial infarction model. / Watanabe, Eiichi; Smith, Duane M.; Delcarpio, Joseph B.; Sun, Jian; Smart, Frank W.; Van Meter, Clifford H.; Claycomb, William C.

In: Cell Transplantation, Vol. 7, No. 3, 01.05.1998, p. 239-246.

Research output: Contribution to journalArticle

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