Catecholamines and serotonin are differently regulated by tetrahydrobiopterin. A study from 6-pyruvoyltetrahydropterin synthase knockout mice

Chiho Sumi-Ichinose, Fumi Urano, Risa Kuroda, Tamae Ohye, Masayo Kojima, Masahiro Tazawa, Hiroaki Shiraishi, Yasumichi Hagino, Toshiharu Nagatsu, Takahide Nomura, Hiroshi Ichinose

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

(6R)-L-erythro-5,6,7,8-Tetrahydrobiopterin (BH4) is an essential cofactor for tyrosine hydroxylase (TH), tryptophan hydroxylase, phenylalanine hydroxylase, and nitric-oxide synthase. These enzymes synthesize neurotransmitters, e.g. catecholamines, serotonin, and nitric oxide (NO). We established mice unable to synthesize BH4 by disruption of the 6-pyruvoyltetrahydropterin synthase gene, the encoded protein of which catalyzes the second step of BH4 biosynthesis. Homozygous mice were born at the almost expected Mendelian ratio, but died within 48 h after birth. In the brain of homozygous mutant neonates, levels of biopterin, catecholamines, and serotonin were extremely low. The number of TH molecules was highly dependent on the intracellular concentration of BH4 at nerve terminals. Alteration of the TH protein level by modulation of the BH4 content is a novel regulatory mechanism. Our data showing that catecholaminergic, serotonergic, and NO systems were differently affected by BH4 starvation suggest the possible involvement of BH4 synthesis in the etiology of monoamine-based neurological and neuropsychiatric disorders.

Original languageEnglish
Pages (from-to)41150-41160
Number of pages11
JournalJournal of Biological Chemistry
Volume276
Issue number44
DOIs
Publication statusPublished - 02-11-2001

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Tyrosine 3-Monooxygenase
Knockout Mice
Catecholamines
Serotonin
Nitric Oxide
Biopterin
Phenylalanine Hydroxylase
Tryptophan Hydroxylase
Biosynthesis
Starvation
Nervous System Diseases
Nitric Oxide Synthase
Neurotransmitter Agents
Brain
Proteins
Genes
Modulation
Parturition
Molecules
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sumi-Ichinose, Chiho ; Urano, Fumi ; Kuroda, Risa ; Ohye, Tamae ; Kojima, Masayo ; Tazawa, Masahiro ; Shiraishi, Hiroaki ; Hagino, Yasumichi ; Nagatsu, Toshiharu ; Nomura, Takahide ; Ichinose, Hiroshi. / Catecholamines and serotonin are differently regulated by tetrahydrobiopterin. A study from 6-pyruvoyltetrahydropterin synthase knockout mice. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 44. pp. 41150-41160.
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Catecholamines and serotonin are differently regulated by tetrahydrobiopterin. A study from 6-pyruvoyltetrahydropterin synthase knockout mice. / Sumi-Ichinose, Chiho; Urano, Fumi; Kuroda, Risa; Ohye, Tamae; Kojima, Masayo; Tazawa, Masahiro; Shiraishi, Hiroaki; Hagino, Yasumichi; Nagatsu, Toshiharu; Nomura, Takahide; Ichinose, Hiroshi.

In: Journal of Biological Chemistry, Vol. 276, No. 44, 02.11.2001, p. 41150-41160.

Research output: Contribution to journalArticle

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T1 - Catecholamines and serotonin are differently regulated by tetrahydrobiopterin. A study from 6-pyruvoyltetrahydropterin synthase knockout mice

AU - Sumi-Ichinose, Chiho

AU - Urano, Fumi

AU - Kuroda, Risa

AU - Ohye, Tamae

AU - Kojima, Masayo

AU - Tazawa, Masahiro

AU - Shiraishi, Hiroaki

AU - Hagino, Yasumichi

AU - Nagatsu, Toshiharu

AU - Nomura, Takahide

AU - Ichinose, Hiroshi

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