TY - JOUR
T1 - CD36 provides host protection against klebsiella pneumoniae intrapulmonary infection by enhancing lipopolysaccharide responsiveness and macrophage phagocytosis
AU - Olonisakin, Tolani F.
AU - Li, Huihua
AU - Xiong, Zeyu
AU - Kochman, Elizabeth J.K.
AU - Yu, Minting
AU - Qu, Yanyan
AU - Hulver, Mei
AU - Kolls, Jay K.
AU - St Croix, Claudette
AU - Doi, Yohei
AU - Nguyen, Minh Hong
AU - Shanks, Robert M.Q.
AU - Mallampalli, Rama K.
AU - Kagan, Valerian E.
AU - Ray, Anuradha
AU - Silverstein, Roy L.
AU - Ray, Prabir
AU - Lee, Janet S.
N1 - Funding Information:
Financial support. Thisworkwas supported by the Howard Hughes Medical Institute (research fellowaward toT. F.O.), the National Institutes ofHealth (grants R01HL086884[toJ.S.L.],R21AI119042 [toJ.S.L.], andP01HL114453 [to R. K. M. and P. R.]), the Flight Attendant Medical Research Institute (to J. S. L.), the Vascular Medicine Institute, the Hemophilia Center of Western Pennsylvania, and the Institute for Transfusion Medicine (J. S. L.).
Publisher Copyright:
© The Author 2016.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. K. pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified self ligands, is a host determinant of K. pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of 3 distinct K. pneumoniae (hypermucoviscous K1, research K2, and carbapenemase-producing ST258) strains, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K. pneumoniae, regardless of strain. We demonstrate that CD36 enhances LPS responsiveness to K. pneumoniae to increase downstream cytokine production and macrophage phagocytosis that is independent of polysaccharide capsular antigen. Our study provides new insights into host determinants of K. pneumoniae pathogenicity and raises the possibility that functional mutations in CD36 may predispose individuals to K. pneumoniae syndromes.
AB - Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. K. pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified self ligands, is a host determinant of K. pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of 3 distinct K. pneumoniae (hypermucoviscous K1, research K2, and carbapenemase-producing ST258) strains, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K. pneumoniae, regardless of strain. We demonstrate that CD36 enhances LPS responsiveness to K. pneumoniae to increase downstream cytokine production and macrophage phagocytosis that is independent of polysaccharide capsular antigen. Our study provides new insights into host determinants of K. pneumoniae pathogenicity and raises the possibility that functional mutations in CD36 may predispose individuals to K. pneumoniae syndromes.
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U2 - 10.1093/infdis/jiw451
DO - 10.1093/infdis/jiw451
M3 - Article
C2 - 27683817
AN - SCOPUS:85016050287
VL - 214
SP - 1865
EP - 1875
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 12
ER -