cDNA cloning and expression of human activin βE subunit

Osamu Hashimoto, Kunihiro Tsuchida, Yuuki Ushiro, Yuko Hosoi, Nobuhiko Hoshi, Hiromu Sugino, Yoshihisa Hasegawa

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We cloned human activin βE subunit cDNA from a liver cDNA library using the polymerase chain reaction (PCR) technique. The deduced amino acid sequence was 97 and 96% homologous to the mouse and rat activin βE subunits. Human activin βE subunit tagged with Myc and polyhistidine residues at the COOH terminus was expressed in mammalian cells and secreted into the medium as a disulphide-linked homodimer protein. We also found that the human activin βE protein could bind to follistatin, an activin-binding protein. Northern blot analysis showed that this gene was expressed as a major transcript of 2.7 kb predominantly in human liver. These findings suggest that activin E (dimeric protein) may play a role in humans.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume194
Issue number1-2
DOIs
Publication statusPublished - 30-08-2002

Fingerprint

Activins
Cloning
Organism Cloning
Complementary DNA
Follistatin
Liver
Proteins
Polymerase chain reaction
Gene Library
Disulfides
Northern Blotting
Rats
Amino Acid Sequence
Genes
Cells
Amino Acids
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Hashimoto, O., Tsuchida, K., Ushiro, Y., Hosoi, Y., Hoshi, N., Sugino, H., & Hasegawa, Y. (2002). cDNA cloning and expression of human activin βE subunit. Molecular and Cellular Endocrinology, 194(1-2), 117-122. https://doi.org/10.1016/S0303-7207(02)00157-0
Hashimoto, Osamu ; Tsuchida, Kunihiro ; Ushiro, Yuuki ; Hosoi, Yuko ; Hoshi, Nobuhiko ; Sugino, Hiromu ; Hasegawa, Yoshihisa. / cDNA cloning and expression of human activin βE subunit. In: Molecular and Cellular Endocrinology. 2002 ; Vol. 194, No. 1-2. pp. 117-122.
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Hashimoto, O, Tsuchida, K, Ushiro, Y, Hosoi, Y, Hoshi, N, Sugino, H & Hasegawa, Y 2002, 'cDNA cloning and expression of human activin βE subunit', Molecular and Cellular Endocrinology, vol. 194, no. 1-2, pp. 117-122. https://doi.org/10.1016/S0303-7207(02)00157-0

cDNA cloning and expression of human activin βE subunit. / Hashimoto, Osamu; Tsuchida, Kunihiro; Ushiro, Yuuki; Hosoi, Yuko; Hoshi, Nobuhiko; Sugino, Hiromu; Hasegawa, Yoshihisa.

In: Molecular and Cellular Endocrinology, Vol. 194, No. 1-2, 30.08.2002, p. 117-122.

Research output: Contribution to journalArticle

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AU - Hashimoto, Osamu

AU - Tsuchida, Kunihiro

AU - Ushiro, Yuuki

AU - Hosoi, Yuko

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AU - Sugino, Hiromu

AU - Hasegawa, Yoshihisa

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