cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association

K. Furukawa, Hidehito Inagaki, T. Naruge, S. Tabata, T. Tomida, A. Yamaguchi, M. Yoshikuni, Y. Nagahama, Y. Hotta

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

It is well known that cytoskeleton and karyoskeleton proteins are associated with changes in cell shape and with the rearrangement of the dynamic structures involved in cell division and motility. In higher vertebrates, there are three major skeletal protein groups: microfilaments, microtubules and intermediate filaments, each representing a multigene family. Some of these skeletal proteins are expressed in a temporally- and spatially-specific fashion, and they establish cell-specific cytoplasmic and nucleoplasmic organization during development. Here we report the cDNA cloning of a novel 60 kDa skeletal protein from mouse spermatocytes, termed MNS 1 (meiosis-specific nuclear structural protein), whose computer-predicted protein configuration indicates long α-helical coiled-coil domains flanked by non-helical terminal domains. Functional characterization of MNS1 by ectopic expression in culture cells indicated that it is a detergent-and high salt-resistant skeletal protein which is involved in organization of the nuclear or perinuclear architecture. The MNS1 protein is specifically expressed at the pachytene stage during spermatogenesis, so that its function may involve the determination and maintenance of the appropriate nuclear morphology during meiotic prophase.

Original languageEnglish
Pages (from-to)99-113
Number of pages15
JournalChromosome Research
Volume2
Issue number2
DOIs
Publication statusPublished - 01-03-1994

Fingerprint

Meiosis
Organism Cloning
Complementary DNA
Proteins
Pachytene Stage
Microfilament Proteins
Prophase
Spermatocytes
Intermediate Filaments
Cell Shape
Spermatogenesis
Multigene Family
Nuclear Proteins
Cytoskeleton
Microtubules
Cell Division
Detergents
Cell Movement
Vertebrates
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Furukawa, K. ; Inagaki, Hidehito ; Naruge, T. ; Tabata, S. ; Tomida, T. ; Yamaguchi, A. ; Yoshikuni, M. ; Nagahama, Y. ; Hotta, Y. / cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association. In: Chromosome Research. 1994 ; Vol. 2, No. 2. pp. 99-113.
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Furukawa, K, Inagaki, H, Naruge, T, Tabata, S, Tomida, T, Yamaguchi, A, Yoshikuni, M, Nagahama, Y & Hotta, Y 1994, 'cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association', Chromosome Research, vol. 2, no. 2, pp. 99-113. https://doi.org/10.1007/BF01553489

cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association. / Furukawa, K.; Inagaki, Hidehito; Naruge, T.; Tabata, S.; Tomida, T.; Yamaguchi, A.; Yoshikuni, M.; Nagahama, Y.; Hotta, Y.

In: Chromosome Research, Vol. 2, No. 2, 01.03.1994, p. 99-113.

Research output: Contribution to journalArticle

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