C/EBPβ is required for survival of Ly6C- monocytes

Akihiro Tamura, Hideyo Hirai, Asumi Yokota, Naoka Kamio, Atsushi Sato, Tsukimi Shoji, Takahiro Kashiwagi, Yusuke Torikoshi, Yasuo Miura, Daniel G. Tenen, Taira Maekawa

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9 Citations (Scopus)

Abstract

The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) is highly expressed in monocytes/macrophages. However, its roles inmonopoiesis are largely unknown. Here, we investigated the roles of C/EBPβ in monopoiesis. Further subdivision of monocytes revealed that Cebpb messenger RNA was highly upregulated in Ly6C- monocytes in bone marrow. Accordingly, the number of Ly6C- monocytes was significantly reduced in Cebpb-/- mice. Bonemarrow chimera experiments and Mx1-Cre-mediated deletion of Cebpbrevealed a cellintrinsic and monocyte-specific requirement for C/EBPβ in monopoiesis. In Cebpb-/- mice, turnover of Ly6C- monocytes was highly accelerated and apoptosis of Ly6C- monocytes was increased. Expression of Csf1r, which encodes a receptor for macrophage colonystimulating factor, was significantly reducedin Ly6C- monocytes of Cebpb-/- mice. C/EBPβ bound to positive regulatory elements of Csf1r and promoted its transcription. Collectively, these results indicate that C/EBPβ is a critical factor for Ly6C- monocyte survival, at least in part through upregulation of Csf1r.

Original languageEnglish
Pages (from-to)1809-1818
Number of pages10
JournalBlood
Volume130
Issue number16
DOIs
Publication statusPublished - 19-10-2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Tamura, A., Hirai, H., Yokota, A., Kamio, N., Sato, A., Shoji, T., Kashiwagi, T., Torikoshi, Y., Miura, Y., Tenen, D. G., & Maekawa, T. (2017). C/EBPβ is required for survival of Ly6C- monocytes. Blood, 130(16), 1809-1818. https://doi.org/10.1182/blood-2017-03-772962