TY - JOUR
T1 - C/EBPδ is involved in the amplification of early granulocyte precursors during candidemia-induced "emergency" granulopoiesis
AU - Satake, Sakiko
AU - Hirai, Hideyo
AU - Hayashi, Yoshihiro
AU - Shime, Nobuaki
AU - Tamura, Akihiro
AU - Yao, Hisayuki
AU - Yoshioka, Satoshi
AU - Miura, Yasuo
AU - Inaba, Tohru
AU - Fujita, Naohisa
AU - Ashihara, Eishi
AU - Imanishi, Jiro
AU - Sawa, Teiji
AU - Maekawa, Taira
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPβ is required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations (#1-5) according to their levels of c-Kit and Ly-6G expression. After the induction of candidemia, rapid mobilization of mature granulocytes and an increase in early granulocyte precursors accompanied by cell cycle acceleration was followed by a gradual increase in granulocytes originating from the immature populations. Upon infection, C/EBPβ was upregulated at the protein level in all the granulopoietic subpopulations. The rapid increase in immature subpopulations #1 and #2 observed in C/EBPβ knockout mice at 1 d postinfection was attenuated. Candidemia-induced cell cycle acceleration and proliferation of hematopoietic stem/progenitors were also impaired. Taken together, these data suggest that C/EBPβ is involved in the efficient amplification of early granulocyte precursors during candidemia-induced emergency granulopoiesis.
AB - Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages in which C/EBPβ is required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations (#1-5) according to their levels of c-Kit and Ly-6G expression. After the induction of candidemia, rapid mobilization of mature granulocytes and an increase in early granulocyte precursors accompanied by cell cycle acceleration was followed by a gradual increase in granulocytes originating from the immature populations. Upon infection, C/EBPβ was upregulated at the protein level in all the granulopoietic subpopulations. The rapid increase in immature subpopulations #1 and #2 observed in C/EBPβ knockout mice at 1 d postinfection was attenuated. Candidemia-induced cell cycle acceleration and proliferation of hematopoietic stem/progenitors were also impaired. Taken together, these data suggest that C/EBPβ is involved in the efficient amplification of early granulocyte precursors during candidemia-induced emergency granulopoiesis.
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U2 - 10.4049/jimmunol.1103007
DO - 10.4049/jimmunol.1103007
M3 - Article
C2 - 23024276
AN - SCOPUS:84867911588
SN - 0022-1767
VL - 189
SP - 4546
EP - 4555
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -