TY - JOUR
T1 - Ceftibuten-containing agar plate for detecting group B streptococci with reduced penicillin susceptibility (PRGBS)
AU - Kamiya, Chitose
AU - Kimura, Kouji
AU - Doyama, Yo
AU - Miyazaki, Akira
AU - Morimoto, Makiko
AU - Banno, Hirotsugu
AU - Nagano, Noriyuki
AU - Jin, Wanchun
AU - Wachino, Jun ichi
AU - Yamada, Keiko
AU - Arakawa, Yoshichika
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Penicillins remain first-line agents for treatment of group B Streptococcus (. Streptococcus agalactiae; GBS) infections; however, several reports have confirmed the existence of GBS with reduced penicillin susceptibility (PRGBS). Because no selective agar plates for detection of PRGBS are available to date, in this investigation, we developed the selective agar plate for detection of PRGBS. We used 19 genetically well-confirmed PRGBS isolates and 38 penicillin-susceptible GBS isolates identified in Japan. For preparation of trial PRGBS-selective agar plates, we added 1 of antimicrobial agents (among oxacillin, ceftizoxime, and ceftibuten) to a well-established GBS-selective agar plate. Among 12 trial PRGBS-selective agar plates, Muller-Hinton agar containing 128. μg/mL ceftibuten with 5% sheep blood, 8. μg/mL gentamicin, and 12. μg/mL nalidixic acid was the most appropriate selective agar for PRGBS, showing 100% sensitivity and 81.6% specificity. In cases of potential nosocomial spread of PRGBS, the selective agar plate could be useful and reliable.
AB - Penicillins remain first-line agents for treatment of group B Streptococcus (. Streptococcus agalactiae; GBS) infections; however, several reports have confirmed the existence of GBS with reduced penicillin susceptibility (PRGBS). Because no selective agar plates for detection of PRGBS are available to date, in this investigation, we developed the selective agar plate for detection of PRGBS. We used 19 genetically well-confirmed PRGBS isolates and 38 penicillin-susceptible GBS isolates identified in Japan. For preparation of trial PRGBS-selective agar plates, we added 1 of antimicrobial agents (among oxacillin, ceftizoxime, and ceftibuten) to a well-established GBS-selective agar plate. Among 12 trial PRGBS-selective agar plates, Muller-Hinton agar containing 128. μg/mL ceftibuten with 5% sheep blood, 8. μg/mL gentamicin, and 12. μg/mL nalidixic acid was the most appropriate selective agar for PRGBS, showing 100% sensitivity and 81.6% specificity. In cases of potential nosocomial spread of PRGBS, the selective agar plate could be useful and reliable.
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U2 - 10.1016/j.diagmicrobio.2015.04.010
DO - 10.1016/j.diagmicrobio.2015.04.010
M3 - Article
C2 - 25959629
AN - SCOPUS:84930571927
SN - 0732-8893
VL - 82
SP - 269
EP - 273
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -
