Cell surface-expressed moesin-like HDL/apoA-I binding protein promotes cholesterol efflux from human macrophages

Akifumi Matsuyama, Naohiko Sakai, Hisatoyo Hiraoka, Ken Ichi Hirano, Shizuya Yamashita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

HDL and its major component, apolipoprotein A-I (apoA-I), play a central role in reverse cholesterol transport. We recently reported the involvement of a glycosylphos-phatidylinositol anchor (GPI anchor) in the binding of HDL and apoA-I on human macrophages, and purified an 80 kDa HDL/apoA-I binding protein. In the present study, we characterized the GPI-anchored HDL/apoA-I binding protein from macrophages. The HDL/apoA-I binding protein was purified from macrophages and digested with endopeptidase, and the resultant fragments were sequenced. Cholesterol efflux, flow cytometry, immunoblotting, and immunohistochemical analyses were performed to characterize the HDL/apoA-I binding protein. Two parts of seven amino acid sequences completely matched those of moesin. Flow cytometry, immunoblotting, and immunohistochemistry using anti-moesin antibody showed that the HDL/apoA-I binding protein was N-glycosylated and expressed on the cell surface. It was termed moesin-like protein. Treatment of macrophages with anti-moesin antibody blocked the binding of HDL/apoA-I and suppressed cholesterol efflux. The moesin-like protein was exclusively expressed on macrophages and was upregulated by cholesterol loading and cell differentiation. Our results indicate that the moesin-like HDL/apoA-I binding protein is specifically expressed on the surface of human macrophages and promotes cholesterol efflux from macrophages.

Original languageEnglish
Pages (from-to)78-86
Number of pages9
JournalJournal of Lipid Research
Volume47
Issue number1
DOIs
Publication statusPublished - 01-01-2006

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Macrophages
Apolipoprotein A-I
Carrier Proteins
Cholesterol
Flow cytometry
Immunoblotting
Anti-Idiotypic Antibodies
Flow Cytometry
Endopeptidases
cholesterol-binding protein
moesin
Antibodies
Anchors
Cell Differentiation
Amino Acid Sequence
Proteins
Immunohistochemistry
Amino Acids

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Matsuyama, Akifumi ; Sakai, Naohiko ; Hiraoka, Hisatoyo ; Hirano, Ken Ichi ; Yamashita, Shizuya. / Cell surface-expressed moesin-like HDL/apoA-I binding protein promotes cholesterol efflux from human macrophages. In: Journal of Lipid Research. 2006 ; Vol. 47, No. 1. pp. 78-86.
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Cell surface-expressed moesin-like HDL/apoA-I binding protein promotes cholesterol efflux from human macrophages. / Matsuyama, Akifumi; Sakai, Naohiko; Hiraoka, Hisatoyo; Hirano, Ken Ichi; Yamashita, Shizuya.

In: Journal of Lipid Research, Vol. 47, No. 1, 01.01.2006, p. 78-86.

Research output: Contribution to journalArticle

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