Cell-Surface MMP-9 Protein Is a Novel Functional Marker to Identify and Separate Proangiogenic Cells from Early Endothelial Progenitor Cells Derived from CD133+ Cells

Toshie Kanayasu-Toyoda, Takeshi Tanaka, Yutaka Kikuchi, Eriko Uchida, Akifumi Matsuyama, Teruhide Yamaguchi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To develop cell therapies for ischemic diseases, endothelial progenitor cells (EPCs) have been expected to play a pivotal role in vascular regeneration. It is desirable to use a molecular marker that is related to the function of the cells. Here, a quantitative polymerase chain reaction array revealed that early EPCs derived from CD133+ cells exhibited significant expression of MMP-9. Some populations of early EPCs expressed MMP-9 on the cell surface and others did not. We also attempted to separate the proangiogenic fraction from early EPCs derived from CD133+ cells using a functional cell surface marker, and we then analyzed the MMP-9+ and MMP-9- cell fractions. The MMP-9+ cells not only revealed higher invasion ability but also produced a high amount of IL-8. Moreover, the stimulative effect of MMP-9+ cells on angiogenesis in vitro and in vivo was prohibited by anti-IL-8 antibody. These data indicate that MMP-9 is one of the useful cell surface markers for the separation of angiogenic cells. Our treatment of early EPCs with hyaluronidase caused not only a downregulation of cell-surface MMP-9 but also a decrease in invasion ability, indicating that membrane-bound MMP-9, which is one of the useful markers for early EPCs, plays an important role in angiogenesis.

Original languageEnglish
Pages (from-to)1251-1262
Number of pages12
JournalStem Cells
Volume34
Issue number5
DOIs
Publication statusPublished - 01-05-2016

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Matrix Metalloproteinases
Proteins
Interleukin-8
Endothelial Progenitor Cells
Hyaluronoglucosaminidase
Cell Separation
Cell- and Tissue-Based Therapy
Blood Vessels
Regeneration
Down-Regulation
Polymerase Chain Reaction
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Kanayasu-Toyoda, Toshie ; Tanaka, Takeshi ; Kikuchi, Yutaka ; Uchida, Eriko ; Matsuyama, Akifumi ; Yamaguchi, Teruhide. / Cell-Surface MMP-9 Protein Is a Novel Functional Marker to Identify and Separate Proangiogenic Cells from Early Endothelial Progenitor Cells Derived from CD133+ Cells. In: Stem Cells. 2016 ; Vol. 34, No. 5. pp. 1251-1262.
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Cell-Surface MMP-9 Protein Is a Novel Functional Marker to Identify and Separate Proangiogenic Cells from Early Endothelial Progenitor Cells Derived from CD133+ Cells. / Kanayasu-Toyoda, Toshie; Tanaka, Takeshi; Kikuchi, Yutaka; Uchida, Eriko; Matsuyama, Akifumi; Yamaguchi, Teruhide.

In: Stem Cells, Vol. 34, No. 5, 01.05.2016, p. 1251-1262.

Research output: Contribution to journalArticle

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