Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle

Akiyoshi Uezumi, Masashi Nakatani, Madoka Ikemoto-Uezumi, Naoki Yamamoto, Mitsuhiro Morita, Asami Yamaguchi, Harumoto Yamada, Takehiro Kasai, Satoru Masuda, Asako Narita, Yuko Miyagoe-Suzuki, Shin'ichi Takeda, So ichiro Fukada, Ichizo Nishino, Kunihiro Tsuchida

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.

Original languageEnglish
Pages (from-to)263-278
Number of pages16
JournalStem Cell Reports
Volume7
Issue number2
DOIs
Publication statusPublished - 09-08-2016

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Muscle
Membrane Proteins
Skeletal Muscle
Stem Cells
Adipogenesis
Muscles
Mesenchymal Stromal Cells
Muscle Cells
Population
Satellites
Fibrosis
Fats
Stem cells
Infiltration
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Uezumi, Akiyoshi ; Nakatani, Masashi ; Ikemoto-Uezumi, Madoka ; Yamamoto, Naoki ; Morita, Mitsuhiro ; Yamaguchi, Asami ; Yamada, Harumoto ; Kasai, Takehiro ; Masuda, Satoru ; Narita, Asako ; Miyagoe-Suzuki, Yuko ; Takeda, Shin'ichi ; Fukada, So ichiro ; Nishino, Ichizo ; Tsuchida, Kunihiro. / Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle. In: Stem Cell Reports. 2016 ; Vol. 7, No. 2. pp. 263-278.
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Uezumi, A, Nakatani, M, Ikemoto-Uezumi, M, Yamamoto, N, Morita, M, Yamaguchi, A, Yamada, H, Kasai, T, Masuda, S, Narita, A, Miyagoe-Suzuki, Y, Takeda, S, Fukada, SI, Nishino, I & Tsuchida, K 2016, 'Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle', Stem Cell Reports, vol. 7, no. 2, pp. 263-278. https://doi.org/10.1016/j.stemcr.2016.07.004

Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle. / Uezumi, Akiyoshi; Nakatani, Masashi; Ikemoto-Uezumi, Madoka; Yamamoto, Naoki; Morita, Mitsuhiro; Yamaguchi, Asami; Yamada, Harumoto; Kasai, Takehiro; Masuda, Satoru; Narita, Asako; Miyagoe-Suzuki, Yuko; Takeda, Shin'ichi; Fukada, So ichiro; Nishino, Ichizo; Tsuchida, Kunihiro.

In: Stem Cell Reports, Vol. 7, No. 2, 09.08.2016, p. 263-278.

Research output: Contribution to journalArticle

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AU - Uezumi, Akiyoshi

AU - Nakatani, Masashi

AU - Ikemoto-Uezumi, Madoka

AU - Yamamoto, Naoki

AU - Morita, Mitsuhiro

AU - Yamaguchi, Asami

AU - Yamada, Harumoto

AU - Kasai, Takehiro

AU - Masuda, Satoru

AU - Narita, Asako

AU - Miyagoe-Suzuki, Yuko

AU - Takeda, Shin'ichi

AU - Fukada, So ichiro

AU - Nishino, Ichizo

AU - Tsuchida, Kunihiro

PY - 2016/8/9

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N2 - Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.

AB - Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.

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