TY - JOUR
T1 - Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle
AU - Uezumi, Akiyoshi
AU - Nakatani, Masashi
AU - Ikemoto-Uezumi, Madoka
AU - Yamamoto, Naoki
AU - Morita, Mitsuhiro
AU - Yamaguchi, Asami
AU - Yamada, Harumoto
AU - Kasai, Takehiro
AU - Masuda, Satoru
AU - Narita, Asako
AU - Miyagoe-Suzuki, Yuko
AU - Takeda, Shin'ichi
AU - Fukada, So ichiro
AU - Nishino, Ichizo
AU - Tsuchida, Kunihiro
N1 - Publisher Copyright:
© 2016 The Author(s)
PY - 2016/8/9
Y1 - 2016/8/9
N2 - Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.
AB - Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases.
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U2 - 10.1016/j.stemcr.2016.07.004
DO - 10.1016/j.stemcr.2016.07.004
M3 - Article
C2 - 27509136
AN - SCOPUS:84991738585
SN - 2213-6711
VL - 7
SP - 263
EP - 278
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 2
ER -
