Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain

Kazunobu Sawamoto, Yuki Hirota, Clara Alfaro-Cervello, Mario Soriano-Navarro, Xiaoping He, Yoshika Hayakawa-Yano, Masayuki Yamada, Keigo Hikishima, Hidenori Tabata, Akio Iwanami, Kazunori Nakajima, Yoshiaki Toyama, Toshio Itoh, Arturo Alvarez-Buylla, Jose Manuel Garcia-Verdugo, Hideyuki Okano

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Abstract

The adult subventricular zone (SVZ) of the lateral ventricle contains neural stem cells. In rodents, these cells generate neuroblasts that migrate as chains toward the olfactory bulb along the rostral migratory stream (RMS). The neural-stem-cell niche at the ventricular wall is conserved in various animal species, including primates. However, it is unclear how the SVZ and RMS organization in nonhuman primates relates to that of rodents and humans. Here we studied the SVZ and RMS of the adult and neonatal common marmoset (Callithrix jacchus), a New World primate used widely in neuroscience, by electron microscopy, and immunohistochemical detection of cell-type-specific markers. The marmoset SVZ contained cells similar to type B, C, and A cells of the rodent SVZ in their marker expression and morphology. The adult marmoset SVZ had a three-layer organization, as in the human brain, with ependymal, hypocellular, and astrocyte-ribbon layers. However, the hypocellular layer was very thin or absent in the adult-anterior and neonatal SVZ. Anti-PSA-NCAM staining of the anterior SVZ in whole-mount ventricular wall preparations of adult marmosets revealed an extensive network of elongated cell aggregates similar to the neuroblast chains in rodents. Time-lapse recordings of marmoset SVZ explants cultured in Matrigel showed the neuroblasts migrating in chains, like rodent type A cells. These results suggest that some features of neurogenesis and neuronal migration in the SVZ are common to marmosets, humans, and rodents. This basic description of the adult and neonatal marmoset SVZ will be useful for future studies on adult neurogenesis in primates.

Original languageEnglish
Pages (from-to)690-713
Number of pages24
JournalJournal of Comparative Neurology
Volume519
Issue number4
DOIs
Publication statusPublished - 01-03-2011

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Callithrix
Lateral Ventricles
Brain
Rodentia
Primates
Neural Stem Cells
Neurogenesis
Stem Cell Niche
Olfactory Bulb
Neurosciences
Astrocytes
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Sawamoto, K., Hirota, Y., Alfaro-Cervello, C., Soriano-Navarro, M., He, X., Hayakawa-Yano, Y., ... Okano, H. (2011). Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. Journal of Comparative Neurology, 519(4), 690-713. https://doi.org/10.1002/cne.22543
Sawamoto, Kazunobu ; Hirota, Yuki ; Alfaro-Cervello, Clara ; Soriano-Navarro, Mario ; He, Xiaoping ; Hayakawa-Yano, Yoshika ; Yamada, Masayuki ; Hikishima, Keigo ; Tabata, Hidenori ; Iwanami, Akio ; Nakajima, Kazunori ; Toyama, Yoshiaki ; Itoh, Toshio ; Alvarez-Buylla, Arturo ; Garcia-Verdugo, Jose Manuel ; Okano, Hideyuki. / Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. In: Journal of Comparative Neurology. 2011 ; Vol. 519, No. 4. pp. 690-713.
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Sawamoto, K, Hirota, Y, Alfaro-Cervello, C, Soriano-Navarro, M, He, X, Hayakawa-Yano, Y, Yamada, M, Hikishima, K, Tabata, H, Iwanami, A, Nakajima, K, Toyama, Y, Itoh, T, Alvarez-Buylla, A, Garcia-Verdugo, JM & Okano, H 2011, 'Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain', Journal of Comparative Neurology, vol. 519, no. 4, pp. 690-713. https://doi.org/10.1002/cne.22543

Cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain. / Sawamoto, Kazunobu; Hirota, Yuki; Alfaro-Cervello, Clara; Soriano-Navarro, Mario; He, Xiaoping; Hayakawa-Yano, Yoshika; Yamada, Masayuki; Hikishima, Keigo; Tabata, Hidenori; Iwanami, Akio; Nakajima, Kazunori; Toyama, Yoshiaki; Itoh, Toshio; Alvarez-Buylla, Arturo; Garcia-Verdugo, Jose Manuel; Okano, Hideyuki.

In: Journal of Comparative Neurology, Vol. 519, No. 4, 01.03.2011, p. 690-713.

Research output: Contribution to journalArticle

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