Cellular differentiation and development of pyloric mucosal metaplasia in the human gall-bladder

Miyuki Shimizu, Kuniyoshi Kitoh, Yasunobu Fujimitsu, Ken Ichi Inada, Masao Ichinose, Kazumasa Miki, Kenji Yamao, Saburo Nakazawa, Mikihiro Shamoto, Masae Tatematsu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The development of pyloric mucosal metaplasia (PMM) with regard to cellular differentiation in the human gallbladder was studied by mucin staining (paradoxical concanavalin A (Con A), galactose oxidase-Schiff (GOS) and alcian blue (pH 2.5) PAS (AB-PAS) and immunohistochemistry (pepsinogen II (PgII) and SH-9, and proliferating cell nuclear antigen (PCNA). PMM was divided into three stages of development by three-dimensional (3D) computer graphic reconstruction analysis. In the early stage, a transitional zone of PCNA positive cells was observed between areas of SH-9 and/or GOS reactive cells and class III and/or Pg II positive cells in flat monolayered epithelium. In the middle stage, shallow pits became apparent as areas enlarged, with these becoming deeper in the advanced stage, whereby SH-9 and/or GOS reactive cells and class III and/or Pg II positive cells were observed at the upper and lower portion of the pits, respectively, with PCNA-positive cells forming a narrow zone between the two cell populations. Consequently, the structure of PMM gradually resembles that of the normal gastric pyloric mucosa.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalPathology International
Volume46
Issue number4
DOIs
Publication statusPublished - 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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