Centrally administered tuberoinfundibular peptide of 39 residues inhibits arginine vasopressin release in conscious rats

Yoshihisa Sugimura, Takashi Murase, Seiji Ishizaki, Kazushige Tachikawa, Hiroshi Arima, Yoshitaka Miura, Ted B. Usdin, Yutaka Oiso

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Tuberoinfundibular peptide of 39 residues (TIP39) is a recently discovered neuropeptide identified on the basis of its ability to activate the PTH2 receptor, and it is thought to be the brain PTH2 receptor's endogenous ligand. The PTH2 receptor is highly expressed in the hypothalamus, suggesting a role in the modulation of neuroendocrinological functions. PTHrP, which also belongs to the PTH-related peptides family, stimulates arginine vasopressin (AVP) release. In the present study, therefore, we investigated the effect of centrally administered TIP39 on AVP release in conscious rats. Intracerebroventricular administration of TIP39 (10-500 pmol/rat) significantly suppressed the plasma AVP concentration in dehydrated rats, and the maximum effect was obtained 5 min after administration (dehydration with 100 pmol/rat TIP39, 4.32 ± 1.17 pg/ml; vs. control, 8.21 ± 0.70 pg/ml). The plasma AVP increase in response to either hyperosmolality [ip injection of hypertonic saline (HS), 600 mosmol/kg] or hypovolemia [ip injection of polyethylene glycol (PEG)] was also significantly attenuated by an intracerebroventricular injection of TIP39 (HS with 100 pmol/rat TIP39, 2.65 ± 0.52 pg/ml; vs. HS alone, 4.69 ± 0.80 pg/ml; PEG with 100 pmol/rat TIP39, 4.10 ± 0.79 pg/ml; vs. PEG alone, 6.19 ± 0.34 pg/ml). Treatment with naloxone [1.5 mg/rat, sc injection], a nonselective opioid receptor antagonist, significantly reversed the inhibitory effects of TIP39 on AVP release. These results suggest that central TIP39 plays an inhibitory role in the osmoregulation and baroregulation of AVP release and that intrinsic opioid systems are involved in its mechanism.

Original languageEnglish
Pages (from-to)2791-2796
Number of pages6
JournalEndocrinology
Volume144
Issue number7
DOIs
Publication statusPublished - 01-07-2003

Fingerprint

Arginine Vasopressin
Receptor, Parathyroid Hormone, Type 2
Parathyroid Hormone-Related Protein
Injections
Osmoregulation
tuberoinfundibular peptide 39
Hypovolemia
Narcotic Antagonists
Naloxone
Neuropeptides
Dehydration
Opioid Analgesics
Hypothalamus
Ligands
Brain

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Sugimura, Yoshihisa ; Murase, Takashi ; Ishizaki, Seiji ; Tachikawa, Kazushige ; Arima, Hiroshi ; Miura, Yoshitaka ; Usdin, Ted B. ; Oiso, Yutaka. / Centrally administered tuberoinfundibular peptide of 39 residues inhibits arginine vasopressin release in conscious rats. In: Endocrinology. 2003 ; Vol. 144, No. 7. pp. 2791-2796.
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abstract = "Tuberoinfundibular peptide of 39 residues (TIP39) is a recently discovered neuropeptide identified on the basis of its ability to activate the PTH2 receptor, and it is thought to be the brain PTH2 receptor's endogenous ligand. The PTH2 receptor is highly expressed in the hypothalamus, suggesting a role in the modulation of neuroendocrinological functions. PTHrP, which also belongs to the PTH-related peptides family, stimulates arginine vasopressin (AVP) release. In the present study, therefore, we investigated the effect of centrally administered TIP39 on AVP release in conscious rats. Intracerebroventricular administration of TIP39 (10-500 pmol/rat) significantly suppressed the plasma AVP concentration in dehydrated rats, and the maximum effect was obtained 5 min after administration (dehydration with 100 pmol/rat TIP39, 4.32 ± 1.17 pg/ml; vs. control, 8.21 ± 0.70 pg/ml). The plasma AVP increase in response to either hyperosmolality [ip injection of hypertonic saline (HS), 600 mosmol/kg] or hypovolemia [ip injection of polyethylene glycol (PEG)] was also significantly attenuated by an intracerebroventricular injection of TIP39 (HS with 100 pmol/rat TIP39, 2.65 ± 0.52 pg/ml; vs. HS alone, 4.69 ± 0.80 pg/ml; PEG with 100 pmol/rat TIP39, 4.10 ± 0.79 pg/ml; vs. PEG alone, 6.19 ± 0.34 pg/ml). Treatment with naloxone [1.5 mg/rat, sc injection], a nonselective opioid receptor antagonist, significantly reversed the inhibitory effects of TIP39 on AVP release. These results suggest that central TIP39 plays an inhibitory role in the osmoregulation and baroregulation of AVP release and that intrinsic opioid systems are involved in its mechanism.",
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Sugimura, Y, Murase, T, Ishizaki, S, Tachikawa, K, Arima, H, Miura, Y, Usdin, TB & Oiso, Y 2003, 'Centrally administered tuberoinfundibular peptide of 39 residues inhibits arginine vasopressin release in conscious rats', Endocrinology, vol. 144, no. 7, pp. 2791-2796. https://doi.org/10.1210/en.2002-0017

Centrally administered tuberoinfundibular peptide of 39 residues inhibits arginine vasopressin release in conscious rats. / Sugimura, Yoshihisa; Murase, Takashi; Ishizaki, Seiji; Tachikawa, Kazushige; Arima, Hiroshi; Miura, Yoshitaka; Usdin, Ted B.; Oiso, Yutaka.

In: Endocrinology, Vol. 144, No. 7, 01.07.2003, p. 2791-2796.

Research output: Contribution to journalArticle

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T1 - Centrally administered tuberoinfundibular peptide of 39 residues inhibits arginine vasopressin release in conscious rats

AU - Sugimura, Yoshihisa

AU - Murase, Takashi

AU - Ishizaki, Seiji

AU - Tachikawa, Kazushige

AU - Arima, Hiroshi

AU - Miura, Yoshitaka

AU - Usdin, Ted B.

AU - Oiso, Yutaka

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