Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells

Yukihiro Akao, Suzuno Kusakabe, Yoshiko Banno, Mariko Kito, Yoshihito Nakagawa, Keiko Tamiya-Koizumi, Masnori Hattori, Motoshi Sawada, Yoshio Hirabayasi, Nobuko Ohishi, Yoshinori Nozawa

Research output: Contribution to journalArticle

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Abstract

We have investigated to determine the source of ceramide produced during the genotoxic apoptosis induced by the anti-cancer drug, camptothecin (CPT), in human prostate cancer LNCaP cells by measuring the activities of acid and neutral sphingomyelinases (SMase) and by using fumonisinB1 (FB1), the inhibitor of ceramide synthase involving de novo synthesis of ceramide. In contrast to time-dependent elevation of intracellular ceramide level after CPT-treatment, the activities of both SMases were not increased but rather decreased. Instead, pretreatment for 3 h with FB1 (100 μM), an inhibitor of ceramide synthase, almost completely abrogated ceramide accumulation observed in cells exposed to CPT for 18 h. These results indicate that ceramide is produced via de novo pathway but not via sphingomyelin hydrolysis pathway. Furthermore, it is to be noted that the pretreatment with FB1 did not affect the CPT-induced apoptosis as assessed by DNA ladder formation, Hoechst 33342 staining, flow cytometry, and mitochondrial potential thereby leading us to propose that ceramide accumulation is independent of apoptosis in this system.

Original languageEnglish
Pages (from-to)363-370
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number2
DOIs
Publication statusPublished - 01-01-2002

Fingerprint

Camptothecin
Ceramides
Prostatic Neoplasms
Apoptosis
Sphingomyelin Phosphodiesterase
Sphingomyelins
Flow cytometry
Ladders
Hydrolysis
Flow Cytometry
Staining and Labeling
Acids
DNA
Pharmaceutical Preparations
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Akao, Yukihiro ; Kusakabe, Suzuno ; Banno, Yoshiko ; Kito, Mariko ; Nakagawa, Yoshihito ; Tamiya-Koizumi, Keiko ; Hattori, Masnori ; Sawada, Motoshi ; Hirabayasi, Yoshio ; Ohishi, Nobuko ; Nozawa, Yoshinori. / Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 294, No. 2. pp. 363-370.
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abstract = "We have investigated to determine the source of ceramide produced during the genotoxic apoptosis induced by the anti-cancer drug, camptothecin (CPT), in human prostate cancer LNCaP cells by measuring the activities of acid and neutral sphingomyelinases (SMase) and by using fumonisinB1 (FB1), the inhibitor of ceramide synthase involving de novo synthesis of ceramide. In contrast to time-dependent elevation of intracellular ceramide level after CPT-treatment, the activities of both SMases were not increased but rather decreased. Instead, pretreatment for 3 h with FB1 (100 μM), an inhibitor of ceramide synthase, almost completely abrogated ceramide accumulation observed in cells exposed to CPT for 18 h. These results indicate that ceramide is produced via de novo pathway but not via sphingomyelin hydrolysis pathway. Furthermore, it is to be noted that the pretreatment with FB1 did not affect the CPT-induced apoptosis as assessed by DNA ladder formation, Hoechst 33342 staining, flow cytometry, and mitochondrial potential thereby leading us to propose that ceramide accumulation is independent of apoptosis in this system.",
author = "Yukihiro Akao and Suzuno Kusakabe and Yoshiko Banno and Mariko Kito and Yoshihito Nakagawa and Keiko Tamiya-Koizumi and Masnori Hattori and Motoshi Sawada and Yoshio Hirabayasi and Nobuko Ohishi and Yoshinori Nozawa",
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Akao, Y, Kusakabe, S, Banno, Y, Kito, M, Nakagawa, Y, Tamiya-Koizumi, K, Hattori, M, Sawada, M, Hirabayasi, Y, Ohishi, N & Nozawa, Y 2002, 'Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells', Biochemical and Biophysical Research Communications, vol. 294, no. 2, pp. 363-370. https://doi.org/10.1016/S0006-291X(02)00462-X

Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells. / Akao, Yukihiro; Kusakabe, Suzuno; Banno, Yoshiko; Kito, Mariko; Nakagawa, Yoshihito; Tamiya-Koizumi, Keiko; Hattori, Masnori; Sawada, Motoshi; Hirabayasi, Yoshio; Ohishi, Nobuko; Nozawa, Yoshinori.

In: Biochemical and Biophysical Research Communications, Vol. 294, No. 2, 01.01.2002, p. 363-370.

Research output: Contribution to journalArticle

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