Change of mouse CD5+ B1 cells to a macrophage-like morphology induced by gamma interferon and inhibited by interleukin-4

Naoki Koide, Tsuyoshi Sugiyama, Isamu Mori, Mya Mya Mu, Teruaki Hamano, Tomoaki Yoshida, Takashi Yokochi

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12 Citations (Scopus)


The in vitro effects of gamma interferon (IFN-γ) on the mouse CD5+ B1-cell line, TH2.52, a hybridoma between mouse B lymphoma and mouse splenic B cells that expresses a series of B1 markers, were investigated. A significant number of macrophage-like cells appeared in the cultures of TH2.52 cells exposed to IFN-γ these adhering to plastic dishes and exhibiting phagocytic activity. Positive for esterase staining, the macrophage-like cells returned to the original TH2.52 morphology upon removal of IFN-γ. The change was prevented by treatment with SB202190, an inhibitor of p38 mitogen-activated protein (MAP) kinase and by transfection of a p38 MAP kinase dominant-negative mutant. Further, interleukin-4 (IL-4) inhibited IFN-γ-induced phosphorylation of p38 MAP kinase and the appearance of macrophage-like cells. IFN-γ and IL-4 exhibited contradictory actions on morphological change of CD5+ B1 cells into macrophage-like cells. Differential regulation of CD5+ B1 cells by IFN-γ, a Th1 cytokine, and IL-4, a Th2 cytokine, may have clear immunological significance.

Original languageEnglish
Pages (from-to)1169-1174
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Issue number6
Publication statusPublished - 11-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)


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