Abstract
The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.
| Original language | English |
|---|---|
| Pages (from-to) | 72-78 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 762 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 11-07-1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate. / Yamada, Kiyofumi; Senzaki, Kouji; Komori, Yumiko; Nikai, Toshiaki; Sugihara, Hisayoshi; Nabeshima, Toshitaka.
In: Brain Research, Vol. 762, No. 1-2, 11.07.1997, p. 72-78.Research output: Contribution to journal › Article
TY - JOUR
T1 - Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate
AU - Yamada, Kiyofumi
AU - Senzaki, Kouji
AU - Komori, Yumiko
AU - Nikai, Toshiaki
AU - Sugihara, Hisayoshi
AU - Nabeshima, Toshitaka
PY - 1997/7/11
Y1 - 1997/7/11
N2 - The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.
AB - The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.
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U2 - 10.1016/S0006-8993(97)00372-7
DO - 10.1016/S0006-8993(97)00372-7
M3 - Article
VL - 762
SP - 72
EP - 78
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -