Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate

Kiyofumi Yamada, Kouji Senzaki, Yumiko Komori, Toshiaki Nikai, Hisayoshi Sugihara, Toshitaka Nabeshima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.

Original languageEnglish
Pages (from-to)72-78
Number of pages7
JournalBrain Research
Volume762
Issue number1-2
DOIs
Publication statusPublished - 11-07-1997
Externally publishedYes

Fingerprint

Nitrites
Neuroglia
Nitrates
Nitric Oxide
Cerebellum
Arginine
2-Amino-5-phosphonovalerate
Cyclopentanes
Excitatory Amino Acid Antagonists
Dicarboxylic Acids
Dialysis Solutions
NG-Nitroarginine Methyl Ester
Microdialysis
N-Methylaspartate
fluorocitrate

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Yamada, Kiyofumi ; Senzaki, Kouji ; Komori, Yumiko ; Nikai, Toshiaki ; Sugihara, Hisayoshi ; Nabeshima, Toshitaka. / Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate. In: Brain Research. 1997 ; Vol. 762, No. 1-2. pp. 72-78.
@article{d003cc5dcc1b4b28baaf5a710f284256,
title = "Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate",
abstract = "The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.",
author = "Kiyofumi Yamada and Kouji Senzaki and Yumiko Komori and Toshiaki Nikai and Hisayoshi Sugihara and Toshitaka Nabeshima",
year = "1997",
month = "7",
day = "11",
doi = "10.1016/S0006-8993(97)00372-7",
language = "English",
volume = "762",
pages = "72--78",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate. / Yamada, Kiyofumi; Senzaki, Kouji; Komori, Yumiko; Nikai, Toshiaki; Sugihara, Hisayoshi; Nabeshima, Toshitaka.

In: Brain Research, Vol. 762, No. 1-2, 11.07.1997, p. 72-78.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate

AU - Yamada, Kiyofumi

AU - Senzaki, Kouji

AU - Komori, Yumiko

AU - Nikai, Toshiaki

AU - Sugihara, Hisayoshi

AU - Nabeshima, Toshitaka

PY - 1997/7/11

Y1 - 1997/7/11

N2 - The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.

AB - The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.

UR - http://www.scopus.com/inward/record.url?scp=0030850845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030850845&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(97)00372-7

DO - 10.1016/S0006-8993(97)00372-7

M3 - Article

C2 - 9262160

AN - SCOPUS:0030850845

VL - 762

SP - 72

EP - 78

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -