Changes in neuronal protein expression in LP-BM5-infected mice

Kanako Takahashi, Kuniaki Saito, Junichi Masuda, Suwako Fujigaki, Masao Takemura, Hiroyasu Ito, Mitsuru Seishima

Research output: Contribution to journalArticle

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Abstract

Murine acquired immunodeficiency syndrome (MAIDS) induced by LP-BM5 murine leukemia virus is used as a model of human immunodeficiency virus (HIV)-related neurologic dysfunction. Mice infected with LP-BM5 have mnemonic abnormalities (i.e., spontaneous alternation behavior in the Y-maze and performance in the Morris water maze) and biochemical alternations (i.e., cytokines, platelet-activating factor, quinolinate, glutamate and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) that produce neurologic symptoms similar to those observed in HIV-related neurologic dysfunction. To identify proteins associated with dysmnesia in the MAIDS model, we examined the expression of neuronal proteins in LP-BM5-infected mice using two-dimensional polyacrylamide gel electrophoresis (2-DE). Neuronal protein expression in LP-BM5-infected mice was compared with that in non-infected mice using the Image Master 2D. We detected approximately 800 protein spots, of which 35 were distinguishable between non-infected and LP-BM5-infected mice. Most of these spots were downregulated in LP-BM5-infected mice. Three of the spots were identified as 14-3-3 protein zeta/delta, synapsin 2 and protein disulfide isomerase using a capillary nanoliquid chromatography tandem mass spectrometric system. We verified the expression levels of these proteins by Western blot. Analysis of these 35 spots could provide insight into mechanisms of dysmnesia in the MAIDS model of HIV-related neuronal dysfunction.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalNeuroscience Letters
Volume422
Issue number2
DOIs
Publication statusPublished - 11-07-2007

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Murine Acquired Immunodeficiency Syndrome
Neurologic Manifestations
Proteins
HIV
Synapsins
Quinolinic Acid
14-3-3 Proteins
Protein Disulfide-Isomerases
Murine Leukemia Viruses
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Platelet Activating Factor
Electrophoresis, Gel, Two-Dimensional
Chromatography
Glutamic Acid
Down-Regulation
Western Blotting
Cytokines
Water

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Takahashi, K., Saito, K., Masuda, J., Fujigaki, S., Takemura, M., Ito, H., & Seishima, M. (2007). Changes in neuronal protein expression in LP-BM5-infected mice. Neuroscience Letters, 422(2), 114-118. https://doi.org/10.1016/j.neulet.2007.05.059
Takahashi, Kanako ; Saito, Kuniaki ; Masuda, Junichi ; Fujigaki, Suwako ; Takemura, Masao ; Ito, Hiroyasu ; Seishima, Mitsuru. / Changes in neuronal protein expression in LP-BM5-infected mice. In: Neuroscience Letters. 2007 ; Vol. 422, No. 2. pp. 114-118.
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Takahashi, K, Saito, K, Masuda, J, Fujigaki, S, Takemura, M, Ito, H & Seishima, M 2007, 'Changes in neuronal protein expression in LP-BM5-infected mice', Neuroscience Letters, vol. 422, no. 2, pp. 114-118. https://doi.org/10.1016/j.neulet.2007.05.059

Changes in neuronal protein expression in LP-BM5-infected mice. / Takahashi, Kanako; Saito, Kuniaki; Masuda, Junichi; Fujigaki, Suwako; Takemura, Masao; Ito, Hiroyasu; Seishima, Mitsuru.

In: Neuroscience Letters, Vol. 422, No. 2, 11.07.2007, p. 114-118.

Research output: Contribution to journalArticle

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Takahashi K, Saito K, Masuda J, Fujigaki S, Takemura M, Ito H et al. Changes in neuronal protein expression in LP-BM5-infected mice. Neuroscience Letters. 2007 Jul 11;422(2):114-118. https://doi.org/10.1016/j.neulet.2007.05.059