Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A

Lorena López-Cerero, Pilar Egea, Lara Serrano, Dolores Navarro, Azucena Mora, Jorge Blanco, Yohei Doi, David L. Paterson, Jesús Rodríguez-Baño, Alvaro Pascual

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Seven phylogroup A CTX-M-15-producing Escherichia coli isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the fimH gene encoding type 1 fimbriae, and five isolates harboured the iucD gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different HpaI digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured bla CTX-M-15 associated with blaOXA-1, blaTEM, tetA, catB3 and aac(6′)-Ib surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of bla CTX-M-15 as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.

Original languageEnglish
Pages (from-to)365-367
Number of pages3
JournalInternational Journal of Antimicrobial Agents
Volume37
Issue number4
DOIs
Publication statusPublished - 01-04-2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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