Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A

Lorena López-Cerero, Pilar Egea, Lara Serrano, Dolores Navarro, Azucena Mora, Jorge Blanco, Yohei Doi, David L. Paterson, Jesús Rodríguez-Baño, Alvaro Pascual

Research output: Contribution to journalArticle

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Abstract

Seven phylogroup A CTX-M-15-producing Escherichia coli isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the fimH gene encoding type 1 fimbriae, and five isolates harboured the iucD gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different HpaI digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured bla CTX-M-15 associated with blaOXA-1, blaTEM, tetA, catB3 and aac(6′)-Ib surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of bla CTX-M-15 as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.

Original languageEnglish
Pages (from-to)365-367
Number of pages3
JournalInternational Journal of Antimicrobial Agents
Volume37
Issue number4
DOIs
Publication statusPublished - 01-04-2011
Externally publishedYes

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Escherichia coli
Food
Virulence
Genes
Siderophores
Horizontal Gene Transfer
Pulsed Field Gel Electrophoresis
Microbial Drug Resistance
Meat
Digestion
Plasmids
galantide
aerobactin

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

López-Cerero, Lorena ; Egea, Pilar ; Serrano, Lara ; Navarro, Dolores ; Mora, Azucena ; Blanco, Jorge ; Doi, Yohei ; Paterson, David L. ; Rodríguez-Baño, Jesús ; Pascual, Alvaro. / Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A. In: International Journal of Antimicrobial Agents. 2011 ; Vol. 37, No. 4. pp. 365-367.
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Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A. / López-Cerero, Lorena; Egea, Pilar; Serrano, Lara; Navarro, Dolores; Mora, Azucena; Blanco, Jorge; Doi, Yohei; Paterson, David L.; Rodríguez-Baño, Jesús; Pascual, Alvaro.

In: International Journal of Antimicrobial Agents, Vol. 37, No. 4, 01.04.2011, p. 365-367.

Research output: Contribution to journalArticle

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