TY - JOUR
T1 - Characterisation of clinical and food animal Escherichia coli isolates producing CTX-M-15 extended-spectrum β-lactamase belonging to ST410 phylogroup A
AU - López-Cerero, Lorena
AU - Egea, Pilar
AU - Serrano, Lara
AU - Navarro, Dolores
AU - Mora, Azucena
AU - Blanco, Jorge
AU - Doi, Yohei
AU - Paterson, David L.
AU - Rodríguez-Baño, Jesús
AU - Pascual, Alvaro
N1 - Funding Information:
Funding: This work was partially supported by the Ministerio de Ciencia e Innovación (Instituto de Salud Carlos III, Fondo de Investigación Sanitaria, REIPI- RD06/0008-1018 , PS09/01273 and PI070190 ), the Ministerio de Educación y Ciencia ( AGL-2008-02129 ), Junta de Andalucía ( PI-0048/2008 , P09-CTS-5259 ), Xunta de Galicia ( 09TAL007261PR , 2007/000044-0 ) and The European Regional Development Fund (ERDF) . AM acknowledges the Ramón y Cajal programme from the Ministerio de Educación y Ciencia de España.
PY - 2011/4
Y1 - 2011/4
N2 - Seven phylogroup A CTX-M-15-producing Escherichia coli isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the fimH gene encoding type 1 fimbriae, and five isolates harboured the iucD gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different HpaI digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured bla CTX-M-15 associated with blaOXA-1, blaTEM, tetA, catB3 and aac(6′)-Ib surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of bla CTX-M-15 as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.
AB - Seven phylogroup A CTX-M-15-producing Escherichia coli isolates recovered from clinical and meat samples were further characterised. All of them belonged to sequence type ST410. Only 2 of the 22 virulence genes investigated were detected. All isolates carried the fimH gene encoding type 1 fimbriae, and five isolates harboured the iucD gene encoding aerobactin siderophore. A group of five isolates showed 81.2% similarity by pulsed-field gel electrophoresis (PFGE), comprising three clinical isolates belonging to ONT:H9 and two food isolates belonging to O55:H9. Different HpaI digestion patterns were observed for plasmids, but all of them belonged to IncFIB group and harboured bla CTX-M-15 associated with blaOXA-1, blaTEM, tetA, catB3 and aac(6′)-Ib surrounded by an identical genetic environment. These findings showed the possibility of lateral gene transfer of bla CTX-M-15 as well as other antibiotic resistance determinants between low-virulence food and clinical isolates.
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U2 - 10.1016/j.ijantimicag.2011.01.001
DO - 10.1016/j.ijantimicag.2011.01.001
M3 - Article
C2 - 21330111
AN - SCOPUS:79952817871
SN - 0924-8579
VL - 37
SP - 365
EP - 367
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 4
ER -