Characterisation of salmon and trout CD8α and CD8β

L. J. Moore, T. Somamoto, K. K. Lie, J・m Dijkstra, I. Hordvik

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The genes and corresponding cDNAs of both α and β chains of the Atlantic salmon (Salmo salar) CD8 molecule have been sequenced and characterized. In addition, the cDNAs for α and β chains of brown trout (Salmo trutta) and for the β chain in rainbow trout (Oncorhynchus mykiss) have been sequenced. The cDNAs code for signal sequences which are preceded by short 5′ UTRs. These are followed by typical immunoglobulin superfamily variable sequences all of which contain two conserved cysteines for the intra-chain disulphide bond. The hinge regions display conserved cysteines for dimerisation and several O-glycosylation motifs for each predicted protein. The domain sharing the highest sequence identity with mammals is the single pass transmembrane domain for all sequences. In salmon, each domain is predominantly coded for by a single exon except the cytoplasmic/3′ UTR domains, which are coded for by 3 and 2 exons for the alpha and beta genes, respectively. In the alpha gene, the second cytoplasmic exon may be spliced out to form an alternative shorter transcript which if expressed would exhibit a truncated cytoplasmic tail. A splice variant found for the salmon beta gene introduces a stop codon after only 40 amino acids. Overall amino acid identities between salmonid sequences were higher than 90%, whereas they shared only 15-20% identity with species such as, chicken and human. Analysis of the expression patterns of the two salmon genes using quantitative RT-PCR shows a very high expression in the thymus. This is mirrored by the expression of the TCRα gene, which is known to be co-expressed with CD8 on mammalian T cells. This is the first report of a sequence for CD8β in a teleost and together with the CD8α sequence, it encodes the ortholog of the CD8 co-receptor molecule on mammalian T cells.

Original languageEnglish
Pages (from-to)1225-1234
Number of pages10
JournalMolecular Immunology
Volume42
Issue number10
DOIs
Publication statusPublished - 01-01-2005

Fingerprint

Trout
Salmon
Salmo salar
Exons
Complementary DNA
Genes
Oncorhynchus mykiss
Cysteine
T-Lymphocytes
Amino Acids
Terminator Codon
5' Untranslated Regions
Dimerization
3' Untranslated Regions
Protein Sorting Signals
Glycosylation
Disulfides
Thymus Gland
Tail
Immunoglobulins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Moore, L. J. ; Somamoto, T. ; Lie, K. K. ; Dijkstra, J・m ; Hordvik, I. / Characterisation of salmon and trout CD8α and CD8β. In: Molecular Immunology. 2005 ; Vol. 42, No. 10. pp. 1225-1234.
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Moore, LJ, Somamoto, T, Lie, KK, Dijkstra, J & Hordvik, I 2005, 'Characterisation of salmon and trout CD8α and CD8β', Molecular Immunology, vol. 42, no. 10, pp. 1225-1234. https://doi.org/10.1016/j.molimm.2004.11.017

Characterisation of salmon and trout CD8α and CD8β. / Moore, L. J.; Somamoto, T.; Lie, K. K.; Dijkstra, J・m; Hordvik, I.

In: Molecular Immunology, Vol. 42, No. 10, 01.01.2005, p. 1225-1234.

Research output: Contribution to journalArticle

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