Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand

Evidence for bovine-to-human zoonotic transmission

Ratana Tacharoenmuang, Satoshi Komoto, Ratigorn Guntapong, Tomihiko Ide, Phakapun Singchai, Sompong Upachai, Saori Fukuda, Yumika Yoshida, Takayuki Murata, Tetsushi Yoshikawa, Kriangsak Ruchusatsawat, Kazushi Motomura, Naokazu Takeda, Somchai Sangkitporn, Koki Taniguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An unusual rotavirus strain, DB2015-066 with the G10P[14] genotype (RVA/Human-wt/THA/DB2015-066/2015/G10P[14]), was detected in a stool sample from a child hospitalized with acute gastroenteritis in Thailand. Here, we sequenced and characterized the full-genome of the strain DB2015-066. On whole genomic analysis, strain DB2015-066 was shown to have a unique genotype constellation: G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The backbone genes of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) are commonly found in rotavirus strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain DB2015-066 could be of artiodactyl (likely bovine) origin. Thus, strain DB2015-066 appeared to be derived from through zoonotic transmission of a bovine rotavirus strain. Of note, the VP7 gene of strain DB2015-066 was located in G10 lineage-6 together with ones of bovine and bovine-like rotavirus strains, away from the clusters comprising other G10P[14] strains in G10 lineage-2/4/5/9, suggesting the occurrence of independent bovine-to-human interspecies transmission events. Our observations provide important insights into the origins of rare G10P[14] strains, and into dynamic interactions between artiodactyl and human rotavirus strains.

Original languageEnglish
Pages (from-to)43-57
Number of pages15
JournalInfection, Genetics and Evolution
Volume63
DOIs
Publication statusPublished - 01-09-2018

Fingerprint

Rotavirus
Zoonoses
Thailand
cattle
gene
genotype
gastroenteritis
Genotype
strain analysis
Genes
Tacrine
Hospitalized Child
Gastroenteritis
genomics
genome
bovine
phylogenetics
Genome
genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Tacharoenmuang, Ratana ; Komoto, Satoshi ; Guntapong, Ratigorn ; Ide, Tomihiko ; Singchai, Phakapun ; Upachai, Sompong ; Fukuda, Saori ; Yoshida, Yumika ; Murata, Takayuki ; Yoshikawa, Tetsushi ; Ruchusatsawat, Kriangsak ; Motomura, Kazushi ; Takeda, Naokazu ; Sangkitporn, Somchai ; Taniguchi, Koki. / Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand : Evidence for bovine-to-human zoonotic transmission. In: Infection, Genetics and Evolution. 2018 ; Vol. 63. pp. 43-57.
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abstract = "An unusual rotavirus strain, DB2015-066 with the G10P[14] genotype (RVA/Human-wt/THA/DB2015-066/2015/G10P[14]), was detected in a stool sample from a child hospitalized with acute gastroenteritis in Thailand. Here, we sequenced and characterized the full-genome of the strain DB2015-066. On whole genomic analysis, strain DB2015-066 was shown to have a unique genotype constellation: G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The backbone genes of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) are commonly found in rotavirus strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain DB2015-066 could be of artiodactyl (likely bovine) origin. Thus, strain DB2015-066 appeared to be derived from through zoonotic transmission of a bovine rotavirus strain. Of note, the VP7 gene of strain DB2015-066 was located in G10 lineage-6 together with ones of bovine and bovine-like rotavirus strains, away from the clusters comprising other G10P[14] strains in G10 lineage-2/4/5/9, suggesting the occurrence of independent bovine-to-human interspecies transmission events. Our observations provide important insights into the origins of rare G10P[14] strains, and into dynamic interactions between artiodactyl and human rotavirus strains.",
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Tacharoenmuang, R, Komoto, S, Guntapong, R, Ide, T, Singchai, P, Upachai, S, Fukuda, S, Yoshida, Y, Murata, T, Yoshikawa, T, Ruchusatsawat, K, Motomura, K, Takeda, N, Sangkitporn, S & Taniguchi, K 2018, 'Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand: Evidence for bovine-to-human zoonotic transmission', Infection, Genetics and Evolution, vol. 63, pp. 43-57. https://doi.org/10.1016/j.meegid.2018.05.009

Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand : Evidence for bovine-to-human zoonotic transmission. / Tacharoenmuang, Ratana; Komoto, Satoshi; Guntapong, Ratigorn; Ide, Tomihiko; Singchai, Phakapun; Upachai, Sompong; Fukuda, Saori; Yoshida, Yumika; Murata, Takayuki; Yoshikawa, Tetsushi; Ruchusatsawat, Kriangsak; Motomura, Kazushi; Takeda, Naokazu; Sangkitporn, Somchai; Taniguchi, Koki.

In: Infection, Genetics and Evolution, Vol. 63, 01.09.2018, p. 43-57.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of a G10P[14] rotavirus strain from a diarrheic child in Thailand

T2 - Evidence for bovine-to-human zoonotic transmission

AU - Tacharoenmuang, Ratana

AU - Komoto, Satoshi

AU - Guntapong, Ratigorn

AU - Ide, Tomihiko

AU - Singchai, Phakapun

AU - Upachai, Sompong

AU - Fukuda, Saori

AU - Yoshida, Yumika

AU - Murata, Takayuki

AU - Yoshikawa, Tetsushi

AU - Ruchusatsawat, Kriangsak

AU - Motomura, Kazushi

AU - Takeda, Naokazu

AU - Sangkitporn, Somchai

AU - Taniguchi, Koki

PY - 2018/9/1

Y1 - 2018/9/1

N2 - An unusual rotavirus strain, DB2015-066 with the G10P[14] genotype (RVA/Human-wt/THA/DB2015-066/2015/G10P[14]), was detected in a stool sample from a child hospitalized with acute gastroenteritis in Thailand. Here, we sequenced and characterized the full-genome of the strain DB2015-066. On whole genomic analysis, strain DB2015-066 was shown to have a unique genotype constellation: G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The backbone genes of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) are commonly found in rotavirus strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain DB2015-066 could be of artiodactyl (likely bovine) origin. Thus, strain DB2015-066 appeared to be derived from through zoonotic transmission of a bovine rotavirus strain. Of note, the VP7 gene of strain DB2015-066 was located in G10 lineage-6 together with ones of bovine and bovine-like rotavirus strains, away from the clusters comprising other G10P[14] strains in G10 lineage-2/4/5/9, suggesting the occurrence of independent bovine-to-human interspecies transmission events. Our observations provide important insights into the origins of rare G10P[14] strains, and into dynamic interactions between artiodactyl and human rotavirus strains.

AB - An unusual rotavirus strain, DB2015-066 with the G10P[14] genotype (RVA/Human-wt/THA/DB2015-066/2015/G10P[14]), was detected in a stool sample from a child hospitalized with acute gastroenteritis in Thailand. Here, we sequenced and characterized the full-genome of the strain DB2015-066. On whole genomic analysis, strain DB2015-066 was shown to have a unique genotype constellation: G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The backbone genes of this strain (I2-R2-C2-M2-A3-N2-T6-E2-H3) are commonly found in rotavirus strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain DB2015-066 could be of artiodactyl (likely bovine) origin. Thus, strain DB2015-066 appeared to be derived from through zoonotic transmission of a bovine rotavirus strain. Of note, the VP7 gene of strain DB2015-066 was located in G10 lineage-6 together with ones of bovine and bovine-like rotavirus strains, away from the clusters comprising other G10P[14] strains in G10 lineage-2/4/5/9, suggesting the occurrence of independent bovine-to-human interspecies transmission events. Our observations provide important insights into the origins of rare G10P[14] strains, and into dynamic interactions between artiodactyl and human rotavirus strains.

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