Characterization of a naturally occurring class D β-lactamase from Achromobacter xylosoxidans

Yohei Doi, Laurent Poirel, David L. Paterson, Patrice Nordmann

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A chromosomally encoded class D β-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. β-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D β-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that blaOXA.114-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this β-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of blaOXA.114 expression.

Original languageEnglish
Pages (from-to)1952-1956
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume52
Issue number6
DOIs
Publication statusPublished - 01-06-2008

Fingerprint

Achromobacter denitrificans
Imipenem
Burkholderia cenocepacia
Cefoxitin
Hydrolysis
Asp(5)-oxytocin
Amino Acids
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Doi, Yohei ; Poirel, Laurent ; Paterson, David L. ; Nordmann, Patrice. / Characterization of a naturally occurring class D β-lactamase from Achromobacter xylosoxidans. In: Antimicrobial agents and chemotherapy. 2008 ; Vol. 52, No. 6. pp. 1952-1956.
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Characterization of a naturally occurring class D β-lactamase from Achromobacter xylosoxidans. / Doi, Yohei; Poirel, Laurent; Paterson, David L.; Nordmann, Patrice.

In: Antimicrobial agents and chemotherapy, Vol. 52, No. 6, 01.06.2008, p. 1952-1956.

Research output: Contribution to journalArticle

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