Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate

Yohei Doi, Naohiro Shibata, Keigo Shibayama, Kazunari Kamachi, Hiroshi Kurokawa, Keiko Yokoyama, Tetsuya Yagi, Yoshichika Arakawa

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

An Escherichia coli strain, HKYM68, which showed resistance to broad-spectrum cephalosporins was isolated from a sputum specimen in Japan. The high-level resistance of the strain to ceftazidime, cefpirome, and moxalactam was carried by a self-transferable plasmid. The β-lactamase gene responsible for the resistance was cloned and sequenced. The deduced amino acid sequence of this gene product, CMY-9, had a single amino acid substitution (E85D), the residue reported to be part of the recognition site for the R1 side chain of β-lactams, compared with the amino acid sequence of CMY-8 and also had 78% identity with the amino acid sequence of CepH, a chromosomal cephalosporinase of Aeromonas hydrophila. A sull-type class 1 integron containing an aacA1-orfG gene cassette was identified upstream of blaCMY-9 and ended with a truncated 3′ conserved segment. The following 2.1 kb was almost identical to the common region of integrons In6 and In7 and the integron of pSAL-1, except that orf513 encoding a putative transposase was identified instead of orf341 due to addition of a single nucleotide. blaCMY-9 was closely located downstream of the end of the common region. These observations are indicative of the exogenous derivation of blaCMY-9 from some environmental microorganisms such as aeromonads.

Original languageEnglish
Pages (from-to)2427-2434
Number of pages8
JournalAntimicrobial agents and chemotherapy
Volume46
Issue number8
DOIs
Publication statusPublished - 01-01-2002

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Cephalosporinase
Integrons
Amino Acid Sequence
Plasmids
cefpirome
Escherichia coli
Moxalactam
Genes
Transposases
Aeromonas hydrophila
Lactams
Ceftazidime
Amino Acid Substitution
Cephalosporins
Sputum
Japan
Nucleotides

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Doi, Yohei ; Shibata, Naohiro ; Shibayama, Keigo ; Kamachi, Kazunari ; Kurokawa, Hiroshi ; Yokoyama, Keiko ; Yagi, Tetsuya ; Arakawa, Yoshichika. / Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate. In: Antimicrobial agents and chemotherapy. 2002 ; Vol. 46, No. 8. pp. 2427-2434.
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Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate. / Doi, Yohei; Shibata, Naohiro; Shibayama, Keigo; Kamachi, Kazunari; Kurokawa, Hiroshi; Yokoyama, Keiko; Yagi, Tetsuya; Arakawa, Yoshichika.

In: Antimicrobial agents and chemotherapy, Vol. 46, No. 8, 01.01.2002, p. 2427-2434.

Research output: Contribution to journalArticle

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