Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins

Naïma Ismaïli, David Pérez-Morga, Patrick Walsh, Akira Maeda, Annette Pays, Patricia Tebabi, Adrian R. Krainer, Etienne Pays

Research output: Contribution to journalArticle

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Abstract

The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery from higher eukaryotes. Moreover, in the yeast two-hybrid system, TSR1 is able to interact with the human splicing factors involved in the recognition of the 3' splicing site (U2AF35/U2AF65). In both procyclic and bloodstream forms of T. brucei, TSR1 was found to localize in the nucleus. In the bloodstream stage TSR1 showed the speckles pattern characteristic of SR proteins involved in cis-splicing. Moreover, TSR1 was able to specifically bind the spliced leader (SL) RNA involved in trans-splicing in trypanosomes by the yeast three-hybrid system. These and other observations suggest that TSR1 may be involved in trans-splicing in T. brucei. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)103-115
Number of pages13
JournalMolecular and Biochemical Parasitology
Volume102
Issue number1
DOIs
Publication statusPublished - 30-07-1999

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Trans-Splicing
Protein Splicing
Trypanosoma brucei brucei
Serine
Arginine
Two-Hybrid System Techniques
RNA
Spliced Leader RNA
Proteins
Trypanosomiasis
Eukaryota
Parasites
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

Cite this

Ismaïli, Naïma ; Pérez-Morga, David ; Walsh, Patrick ; Maeda, Akira ; Pays, Annette ; Tebabi, Patricia ; Krainer, Adrian R. ; Pays, Etienne. / Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins. In: Molecular and Biochemical Parasitology. 1999 ; Vol. 102, No. 1. pp. 103-115.
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Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins. / Ismaïli, Naïma; Pérez-Morga, David; Walsh, Patrick; Maeda, Akira; Pays, Annette; Tebabi, Patricia; Krainer, Adrian R.; Pays, Etienne.

In: Molecular and Biochemical Parasitology, Vol. 102, No. 1, 30.07.1999, p. 103-115.

Research output: Contribution to journalArticle

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