TY - JOUR
T1 - Characterization of adenosine deaminase (ADA)‐negative B‐lymphoblastoid cells cocultured with ADA‐positive fibroblasts
AU - Fujita, Masahiro
AU - Ito, Kazuhiko
AU - Kawamoto, Hiroshi
AU - Kashii, Saburo
AU - Norioka, Mihoko
AU - Monden, Sumie
AU - Okuma, Minoru
PY - 1993/4
Y1 - 1993/4
N2 - Abstract: A cell line, BAD05, derived from B lymphocytes of an adenosine deaminase (ADA; EC 3,5,4,4)‐deficient patient could not proliferate in a serum‐free medium containing 100 μmol/l deoxyadenosine. When BAD05 was cultured with ADA‐positive fibroblasts, the proliferation of BAD05 was improved. BAD05 cell density increased when the initially mixed ratio of fibroblasts/BAD05 was 1/10 or higher, but decreased when the ratio was 1/20 or lower. Deoxyadenosine concentrations in the medium and ATP and deoxyATP (dATP) levels in the BAD05 were measured after 4 hours of coculture at initial BAD05 cell densities of 1 × 105and 1 × 106cells/ml. Deoxyadenosine concentrations in the medium decreased as the density of fibroblasts increased. The dATP level decreased as the mixed ratio rose. The ratio of fibroblasts/BAD05 rather than the cell density of fibroblasts had a larger effect on the dATP levels in BAD05. Under our experimental conditions, ADA‐negative cells proliferated well when the ratio of ADA‐positive cells/ADA‐negative cells was over 1/10.
AB - Abstract: A cell line, BAD05, derived from B lymphocytes of an adenosine deaminase (ADA; EC 3,5,4,4)‐deficient patient could not proliferate in a serum‐free medium containing 100 μmol/l deoxyadenosine. When BAD05 was cultured with ADA‐positive fibroblasts, the proliferation of BAD05 was improved. BAD05 cell density increased when the initially mixed ratio of fibroblasts/BAD05 was 1/10 or higher, but decreased when the ratio was 1/20 or lower. Deoxyadenosine concentrations in the medium and ATP and deoxyATP (dATP) levels in the BAD05 were measured after 4 hours of coculture at initial BAD05 cell densities of 1 × 105and 1 × 106cells/ml. Deoxyadenosine concentrations in the medium decreased as the density of fibroblasts increased. The dATP level decreased as the mixed ratio rose. The ratio of fibroblasts/BAD05 rather than the cell density of fibroblasts had a larger effect on the dATP levels in BAD05. Under our experimental conditions, ADA‐negative cells proliferated well when the ratio of ADA‐positive cells/ADA‐negative cells was over 1/10.
UR - http://www.scopus.com/inward/record.url?scp=0027309164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027309164&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0609.1993.tb01921.x
DO - 10.1111/j.1600-0609.1993.tb01921.x
M3 - Article
C2 - 8500601
AN - SCOPUS:0027309164
SN - 0902-4441
VL - 50
SP - 200
EP - 205
JO - European Journal of Haematology
JF - European Journal of Haematology
IS - 4
ER -