TY - JOUR
T1 - Characterization of aged rat vocal fold fibroblasts
AU - Kawai, Yoshitaka
AU - Kishimoto, Yo
AU - Sogami, Tohru
AU - Suzuki, Ryo
AU - Tsuji, Takuya
AU - Hiwatashi, Nao
AU - Tateya, Ichiro
AU - Kanemaru, Shin Ichi
AU - Nakamura, Tatsuo
AU - Omori, Koichi
AU - Hirano, Shigeru
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science (grant #25462680 to S.H.).
Publisher Copyright:
© 2018 The American Laryngological, Rhinological and Otological Society, Inc.
PY - 2019/3
Y1 - 2019/3
N2 - Objectives/Hypothesis: To elucidate the aging physiology of the vocal folds, we examined the characters of aged vocal fold fibroblasts (VFFs) in various conditions. Study Design: In vitro study. Methods: VFFs from young (12-week-old) and aged (19-month-old) Sprague-Dawley rats were compared. Proliferative capacity, ratio of myofibroblast to fibroblast, myofibroblast function, and extracellular matrix production were examined in the following conditions: naïve, basic fibroblast growth factor (bFGF) supplemented, and hepatocyte growth factor (HGF) supplemented. Results: Aged VFFs demonstrated reduced proliferation by cell counting, though the ratio of Ki-67–positive cells showed no difference. Aged VFFs exhibited an increased expression of α-smooth muscle actin (α-SMA); however, they demonstrated no enhanced contractile ability in a gel contraction assay. Type I collagen protein was increased age dependently, accompanied with decreased Mmp1 and unchanged Col1a1 transcription. Type I collagen protein and α-SMA represented quite similar reduction patterns to bFGF or HGF administration. Conclusions: The following possible characteristics of aged VFFs were implied: long duration of mitosis, increased myofibroblast population size with certain dysfunctions, reduced type I collagen turnover, and correlation between α-SMA expression and type I collagen metabolism. Further investigations of these features will help to clarify presbyphonia's pathology and establish treatment strategies. Level of Evidence: NA Laryngoscope, 129:E94–E101, 2019.
AB - Objectives/Hypothesis: To elucidate the aging physiology of the vocal folds, we examined the characters of aged vocal fold fibroblasts (VFFs) in various conditions. Study Design: In vitro study. Methods: VFFs from young (12-week-old) and aged (19-month-old) Sprague-Dawley rats were compared. Proliferative capacity, ratio of myofibroblast to fibroblast, myofibroblast function, and extracellular matrix production were examined in the following conditions: naïve, basic fibroblast growth factor (bFGF) supplemented, and hepatocyte growth factor (HGF) supplemented. Results: Aged VFFs demonstrated reduced proliferation by cell counting, though the ratio of Ki-67–positive cells showed no difference. Aged VFFs exhibited an increased expression of α-smooth muscle actin (α-SMA); however, they demonstrated no enhanced contractile ability in a gel contraction assay. Type I collagen protein was increased age dependently, accompanied with decreased Mmp1 and unchanged Col1a1 transcription. Type I collagen protein and α-SMA represented quite similar reduction patterns to bFGF or HGF administration. Conclusions: The following possible characteristics of aged VFFs were implied: long duration of mitosis, increased myofibroblast population size with certain dysfunctions, reduced type I collagen turnover, and correlation between α-SMA expression and type I collagen metabolism. Further investigations of these features will help to clarify presbyphonia's pathology and establish treatment strategies. Level of Evidence: NA Laryngoscope, 129:E94–E101, 2019.
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U2 - 10.1002/lary.27464
DO - 10.1002/lary.27464
M3 - Article
C2 - 30450675
AN - SCOPUS:85057063095
SN - 0023-852X
VL - 129
SP - E94-E101
JO - Laryngoscope
JF - Laryngoscope
IS - 3
ER -