Characterization of cell fusion in XC cells induced by Suncus murinus mammary tumor virus

T. Yokoi, A. Aoyama, S. Nagayoshi, Y. Tsutsui, S. Saga, K. Iida, M. Takahashi, Y. Kimura, M. Hoshino

Research output: Contribution to journalArticle

Abstract

Syncytium formation in a rat tumor cell line (XC) induced by Suncus murinus mammary tumor virus (Sm-MTV) was studied. Multinucleate giant cells containing 20-30 nuclei were formed in a monolayer of XC cells by cocultivation with X-ray-irradiated Sm-MTV producing cells (Sm-MT-1). By fluorescent antibody staining, Sm-MTV antigens were demonstrated in the cytoplasm of syncytia, and budding particles and intracytoplasmic A particles were found in syncytial giant cells by electron microscopy. Cell-free supernatant of Sm-MT-1 was capable of inducing syncytia at a much lower incidence than cocultivation of Sm-MT-1 cells. Syncytium formation was completely inhibited when anti-Sm-MTV bovine serum was added to the coculture medium. Pretreatment of XC cells with actinomycin-D caused a partial reduction of Sm-MTV-induced cell fusion, but syncytium formation did occur at a reduced rate even when cellular RNA synthesis was completely inhibited. Dexamethasone increased virus production in Sm-MT-1 cells, resulting in the enhancement of Sm-MTV mediated syncytium formation. Sm-MTV was found to have a unique characteristic of cell fusion activity on XC cells with striking enhancement by dexamethasone.

Original languageEnglish
Pages (from-to)267-276
Number of pages10
JournalArchives of Virology
Volume115
Issue number3-4
DOIs
Publication statusPublished - 01-09-1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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    Yokoi, T., Aoyama, A., Nagayoshi, S., Tsutsui, Y., Saga, S., Iida, K., Takahashi, M., Kimura, Y., & Hoshino, M. (1990). Characterization of cell fusion in XC cells induced by Suncus murinus mammary tumor virus. Archives of Virology, 115(3-4), 267-276. https://doi.org/10.1007/BF01310535