Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism

Kentaro Tsukamoto, Masafumi Mukamoto, Tomoko Kohda, Hideshi Ihara, Xingmin Wang, Tsuneo Maegawa, Shinichi Nakamura, Tadahiro Karasawa, Shunji Kozaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) and C. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalMicrobial Pathogenesis
Volume33
Issue number4
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

Fingerprint

Clostridium butyricum
Botulism
Neurotoxins
Synaptosomal-Associated Protein 25
Food
Synaptic Membranes
Monoclonal Antibodies
Brain

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Tsukamoto, Kentaro ; Mukamoto, Masafumi ; Kohda, Tomoko ; Ihara, Hideshi ; Wang, Xingmin ; Maegawa, Tsuneo ; Nakamura, Shinichi ; Karasawa, Tadahiro ; Kozaki, Shunji. / Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism. In: Microbial Pathogenesis. 2002 ; Vol. 33, No. 4. pp. 177-184.
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Tsukamoto, K, Mukamoto, M, Kohda, T, Ihara, H, Wang, X, Maegawa, T, Nakamura, S, Karasawa, T & Kozaki, S 2002, 'Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism', Microbial Pathogenesis, vol. 33, no. 4, pp. 177-184. https://doi.org/10.1016/S0882-4010(02)90525-5

Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism. / Tsukamoto, Kentaro; Mukamoto, Masafumi; Kohda, Tomoko; Ihara, Hideshi; Wang, Xingmin; Maegawa, Tsuneo; Nakamura, Shinichi; Karasawa, Tadahiro; Kozaki, Shunji.

In: Microbial Pathogenesis, Vol. 33, No. 4, 01.01.2002, p. 177-184.

Research output: Contribution to journalArticle

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AU - Kozaki, Shunji

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