Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism

Kentaro Tsukamoto; Masafumi Mukamoto; Tomoko Kohda; Hideshi Ihara; Xingmin Wang; Tsuneo Maegawa; Shinichi Nakamura; Tadahiro Karasawa; Shunji Kozaki

doi:10.1016/S0882-4010(02)90525-5

Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism

Kentaro Tsukamoto, Masafumi Mukamoto, Tomoko Kohda, Hideshi Ihara, Xingmin Wang, Tsuneo Maegawa, Shinichi Nakamura, Tadahiro Karasawa, Shunji Kozaki

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) and C. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.

Original language	English
Pages (from-to)	177-184
Number of pages	8
Journal	Microbial Pathogenesis
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/S0882-4010(02)90525-5
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0882-4010(02)90525-5

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title = "Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism",

abstract = "The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) and C. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.",

author = "Kentaro Tsukamoto and Masafumi Mukamoto and Tomoko Kohda and Hideshi Ihara and Xingmin Wang and Tsuneo Maegawa and Shinichi Nakamura and Tadahiro Karasawa and Shunji Kozaki",

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doi = "10.1016/S0882-4010(02)90525-5",

language = "English",

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pages = "177--184",

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T1 - Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism

AU - Tsukamoto, Kentaro

AU - Mukamoto, Masafumi

AU - Kohda, Tomoko

AU - Ihara, Hideshi

AU - Wang, Xingmin

AU - Maegawa, Tsuneo

AU - Nakamura, Shinichi

AU - Karasawa, Tadahiro

AU - Kozaki, Shunji

PY - 2002

Y1 - 2002

N2 - The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) and C. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.

AB - The neurotoxin of Clostridium butyricum strain LCL155 (BuNT/LCL155) associated with type E food-borne botulism showed antigenic and biological properties different from those of C. botulinum type E (BoNT/E) and C. butyricum strain BL5262 (BuNT/BL5262). The specific toxicity of BuNT/LCL155 was found to be about 10% those of BoNT/E and BuNT/BL5262. Immunological analysis with monoclonal antibodies against BoNT/E showed that the heavy chain of BuNT/LCL155 differs partially from those of BoNT/E and BuNT/BL5262. Binding experiments with rat brain synaptic membrane revealed that BuNT/LCL155 possesses a binding activity lower than either BoNT/E or BuNT/BL5262. There was no difference in the catalytic activity of the three neurotoxins, which had been determined with a recombinant of the intracellular target protein SNAP-25. These data suggest that the BuNT/LCL155 shares the receptor-recognition site structurally different from BoNT/E and BuNT/BL5262, perhaps causing a decreased specific toxicity.

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Characterization of Clostridium butyricum neurotoxin associated with food-borne botulism

Abstract

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