Characterization of DNA rearrangements of N-myc gene amplification in three neuroblastoma cell lines by pulsed-field gel electrophoresis

Hiroshi Kato, Kazuhiko Okamura, Yoshikazu Kurosawa, Teruaki Kishikawa, Keiichiro Hashimoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We characterized N-myc gene amplification in three human neuroblastoma cell lines (IMR-32, TGW, GOTO). Rearrangements in long-range regions surrounding amplified N-myc genes were examined by pulsed-field gel electrophoresis. Since rare-cutting enzymes completely digested DNA at the middle of the N-myc gene, we were able to construct a physical map upstream and downstream of the germline N-myc gene, and to obtain information on restriction sites surrounding amplified N-myc genes. This method enables us to envisage the organization of amplified units over a long range. Digestion patterns differed considerably among the germline and the three cell lines, but were simple in each case. We estimated that the minimal distance between neighboring N-myc genes is at least several hundred kilobases. Our data suggest that amplification units contain several DNA fragments derived from ditterent loci, but that they are homogeneous.

Original languageEnglish
Pages (from-to)529-535
Number of pages7
JournalFEBS Letters
Volume250
Issue number2
DOIs
Publication statusPublished - 03-07-1989

Fingerprint

myc Genes
Gene Rearrangement
Gene Amplification
Pulsed Field Gel Electrophoresis
Electrophoresis
Neuroblastoma
Genes
Gels
Cells
Cell Line
DNA
Amplification
Digestion
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kato, Hiroshi ; Okamura, Kazuhiko ; Kurosawa, Yoshikazu ; Kishikawa, Teruaki ; Hashimoto, Keiichiro. / Characterization of DNA rearrangements of N-myc gene amplification in three neuroblastoma cell lines by pulsed-field gel electrophoresis. In: FEBS Letters. 1989 ; Vol. 250, No. 2. pp. 529-535.
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Characterization of DNA rearrangements of N-myc gene amplification in three neuroblastoma cell lines by pulsed-field gel electrophoresis. / Kato, Hiroshi; Okamura, Kazuhiko; Kurosawa, Yoshikazu; Kishikawa, Teruaki; Hashimoto, Keiichiro.

In: FEBS Letters, Vol. 250, No. 2, 03.07.1989, p. 529-535.

Research output: Contribution to journalArticle

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T1 - Characterization of DNA rearrangements of N-myc gene amplification in three neuroblastoma cell lines by pulsed-field gel electrophoresis

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AU - Hashimoto, Keiichiro

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