Characterization of invivo tumorigenicity tests using severe immunodeficient NOD/Shi-scid IL2Rγnull mice for detection of tumorigenic cellular impurities in human cell-processed therapeutic products

Shinji Kusakawa, Kazuhiko Machida, Satoshi Yasuda, Nozomi Takada, Takuya Kuroda, Rumi Sawada, Hanayuki Okura, Hideki Tsutsumi, Shin Kawamata, Yoji Sato

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The contamination of human cell-processed therapeutic products (hCTPs) with tumorigenic cells is one of the major concerns in the manufacturing and quality control of hCTPs. However, no quantitative method for detecting the tumorigenic cellular impurities is currently standardized. NOD/Shi-scid IL2Rγnull (NOG) mice have shown high xeno-engraftment potential compared with other well-known immunodeficient strains, e.g. nude mice. Hypothesizing that tumorigenicity test using NOG mice could be a sensitive and quantitative method to detect a small amount of tumorigenic cells in hCTPs, we examined tumor formation after subcutaneous transplantation of HeLa cells, as a model of tumorigenic cells, in NOG mice and nude mice. Sixteen weeks after inoculation, the 50% tumor-producing dose (TPD50) values of HeLa cells were stable at 1.3×104 and 4.0×105 cells in NOG and nude mice, respectively, indicating a 30-fold higher sensitivity of NOG mice compared to that of nude mice. Transplanting HeLa cells embedded with Matrigel in NOG mice further decreased the TPD50 value to 7.9×10 cells, leading to a 5000-fold higher sensitivity, compared with that of nude mice. Additionally, when HeLa cells were mixed with 106 or 107 human mesenchymal stem cells as well as Matrigel, the TPD50 values in NOG mice were comparable to those of HeLa cells alone with Matrigel. These results suggest that the invivo tumorigenicity test using NOG mice with Matrigel is a highly sensitive and quantitative method to detect a trace amount of tumorigenic cellular impurities in human somatic cells, which can be useful in the quality assessment of hCTPs.

Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalRegenerative Therapy
Volume1
DOIs
Publication statusPublished - 01-06-2015

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Carcinogenicity Tests
Tumors
Cells
Impurities
Stem cells
HeLa Cells
Nude Mice
Quality control
Contamination
Therapeutics
matrigel
Mesenchymal Stromal Cells
Quality Control
Neoplasms
Transplantation

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

Cite this

Kusakawa, Shinji ; Machida, Kazuhiko ; Yasuda, Satoshi ; Takada, Nozomi ; Kuroda, Takuya ; Sawada, Rumi ; Okura, Hanayuki ; Tsutsumi, Hideki ; Kawamata, Shin ; Sato, Yoji. / Characterization of invivo tumorigenicity tests using severe immunodeficient NOD/Shi-scid IL2Rγnull mice for detection of tumorigenic cellular impurities in human cell-processed therapeutic products. In: Regenerative Therapy. 2015 ; Vol. 1. pp. 30-37.
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abstract = "The contamination of human cell-processed therapeutic products (hCTPs) with tumorigenic cells is one of the major concerns in the manufacturing and quality control of hCTPs. However, no quantitative method for detecting the tumorigenic cellular impurities is currently standardized. NOD/Shi-scid IL2Rγnull (NOG) mice have shown high xeno-engraftment potential compared with other well-known immunodeficient strains, e.g. nude mice. Hypothesizing that tumorigenicity test using NOG mice could be a sensitive and quantitative method to detect a small amount of tumorigenic cells in hCTPs, we examined tumor formation after subcutaneous transplantation of HeLa cells, as a model of tumorigenic cells, in NOG mice and nude mice. Sixteen weeks after inoculation, the 50{\%} tumor-producing dose (TPD50) values of HeLa cells were stable at 1.3×104 and 4.0×105 cells in NOG and nude mice, respectively, indicating a 30-fold higher sensitivity of NOG mice compared to that of nude mice. Transplanting HeLa cells embedded with Matrigel in NOG mice further decreased the TPD50 value to 7.9×10 cells, leading to a 5000-fold higher sensitivity, compared with that of nude mice. Additionally, when HeLa cells were mixed with 106 or 107 human mesenchymal stem cells as well as Matrigel, the TPD50 values in NOG mice were comparable to those of HeLa cells alone with Matrigel. These results suggest that the invivo tumorigenicity test using NOG mice with Matrigel is a highly sensitive and quantitative method to detect a trace amount of tumorigenic cellular impurities in human somatic cells, which can be useful in the quality assessment of hCTPs.",
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Characterization of invivo tumorigenicity tests using severe immunodeficient NOD/Shi-scid IL2Rγnull mice for detection of tumorigenic cellular impurities in human cell-processed therapeutic products. / Kusakawa, Shinji; Machida, Kazuhiko; Yasuda, Satoshi; Takada, Nozomi; Kuroda, Takuya; Sawada, Rumi; Okura, Hanayuki; Tsutsumi, Hideki; Kawamata, Shin; Sato, Yoji.

In: Regenerative Therapy, Vol. 1, 01.06.2015, p. 30-37.

Research output: Contribution to journalArticle

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AU - Sato, Yoji

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