Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri

Francois Lebreton, Brendan W. Corey, Christi L. McElheny, Alina Iovleva, Lan Preston, Katie R. Margulieux, Robert J. Cybulski, Patrick Mc Gann, Yohei Doi, Jason W. Bennett

Research output: Contribution to journalArticlepeer-review

Abstract

KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that blaKPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala267-Ser275 loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/b-lactamase inhibitor combinations.

Original languageEnglish
Article numbere00150-21
JournalAntimicrobial agents and chemotherapy
Volume65
Issue number7
DOIs
Publication statusPublished - 07-2021

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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