Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri

Francois Lebreton; Brendan W. Corey; Christi L. McElheny; Alina Iovleva; Lan Preston; Katie R. Margulieux; Robert J. Cybulski; Patrick Mc Gann; Yohei Doi; Jason W. Bennett

doi:10.1128/AAC.00150-21

Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri

Francois Lebreton
, Brendan W. Corey
, Christi L. McElheny
, Alina Iovleva
, Lan Preston
, Katie R. Margulieux
, Robert J. Cybulski
, Patrick Mc Gann
, Yohei Doi
, Jason W. Bennett

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that bla_KPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala₂₆₇-Ser₂₇₅ loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/b-lactamase inhibitor combinations.

Original language	English
Article number	e00150-21
Journal	Antimicrobial agents and chemotherapy
Volume	65
Issue number	7
DOIs	https://doi.org/10.1128/AAC.00150-21
Publication status	Published - 07-2021
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00150-21

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Lebreton, F., Corey, B. W., McElheny, C. L., Iovleva, A., Preston, L., Margulieux, K. R., Cybulski, R. J., Gann, P. M., Doi, Y., & Bennett, J. W. (2021). Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri. Antimicrobial agents and chemotherapy, 65(7), Article e00150-21. https://doi.org/10.1128/AAC.00150-21

@article{430bbd60f7484bab928d2a3d53a0d53d,

title = "Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri",

abstract = "KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that blaKPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala267-Ser275 loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/b-lactamase inhibitor combinations.",

keywords = "CRE, Carbapenems, Ceftazidime-avibactam, Citrobacter koseri, KPC",

author = "Francois Lebreton and Corey, \{Brendan W.\} and McElheny, \{Christi L.\} and Alina Iovleva and Lan Preston and Margulieux, \{Katie R.\} and Cybulski, \{Robert J.\} and Gann, \{Patrick Mc\} and Yohei Doi and Bennett, \{Jason W.\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2021 Lebreton et al",

year = "2021",

month = jul,

doi = "10.1128/AAC.00150-21",

language = "English",

volume = "65",

journal = "Antimicrobial agents and chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "7",

}

Lebreton, F, Corey, BW, McElheny, CL, Iovleva, A, Preston, L, Margulieux, KR, Cybulski, RJ, Gann, PM, Doi, Y & Bennett, JW 2021, 'Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri', Antimicrobial agents and chemotherapy, vol. 65, no. 7, e00150-21. https://doi.org/10.1128/AAC.00150-21

Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri. / Lebreton, Francois; Corey, Brendan W.; McElheny, Christi L. et al.
In: Antimicrobial agents and chemotherapy, Vol. 65, No. 7, e00150-21, 07.2021.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri

AU - Lebreton, Francois

AU - Corey, Brendan W.

AU - McElheny, Christi L.

AU - Iovleva, Alina

AU - Preston, Lan

AU - Margulieux, Katie R.

AU - Cybulski, Robert J.

AU - Gann, Patrick Mc

AU - Doi, Yohei

AU - Bennett, Jason W.

PY - 2021/7

Y1 - 2021/7

N2 - KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that blaKPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala267-Ser275 loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/b-lactamase inhibitor combinations.

AB - KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that blaKPC-82 is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala267-Ser275 loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/b-lactamase inhibitor combinations.

KW - CRE

KW - Carbapenems

KW - Ceftazidime-avibactam

KW - Citrobacter koseri

KW - KPC

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UR - https://www.scopus.com/pages/publications/85108274280#tab=citedBy

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DO - 10.1128/AAC.00150-21

M3 - Article

C2 - 33972237

AN - SCOPUS:85108274280

SN - 0066-4804

VL - 65

JO - Antimicrobial agents and chemotherapy

JF - Antimicrobial agents and chemotherapy

IS - 7

M1 - e00150-21

ER -

Characterization of KPC-82, a KPC-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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