Characterization of murine CD160+ CD8+ T lymphocytes

Kunio Tsujimura, Yuichi Obata, Yasue Matsudaira, Keiko Nishida, Yoshiki Akatsuka, Yoshinori Ito, Ayako Demachi-Okamura, Kiyotaka Kuzushima, Toshitada Takahashi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

CD160 is an Ig-like glycoprotein expressed on NK, NKT and TCRγδ T cells, as well as intestinal intraepithelial T lymphocytes. In addition, a minor subset of CD8+ but not CD4+ T cells in the periphery is also known to express CD160, but the subset has not been fully characterized. In this study, we prepared anti-murine CD160 mAbs and investigated the expression profile of CD160 on various subsets of CD8+ T cells. The amount of CD160 on almost all CD8+ T cells was increased upon CD3-mediated stimulation in vitro, and soluble CD160 was found to be released. Flow cytometric analysis revealed most CD8+ T cells expressing CD160 to show a CD44high phenotype in vivo. On further analysis, both CD44highCD62Llow effector memory T cells (TEM) and CD44highCD62Lhigh central memory T cells (TCM) expressed CD160 at an intermediate level. High levels were evident with recently activated CD8+ TEM. Naïve CD8+ T cells presumably immediately after stimulation (CD44lowCD62LlowCD69+) also expressed CD160, but only at a low level. Purified CD160+ CD8+ T cells from OT-1 transgenic mice expressing TCR against OVA residues 257-264 presented by H-2Kb produced IFN-γ more rapidly than CD160- CD8+ T cells upon antigen stimulation. These results together show that CD160 is expressed on the majority of CD8+ memory T cells as well as recently activated CD8+ T cells.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalImmunology Letters
Volume106
Issue number1
DOIs
Publication statusPublished - 15-07-2006
Externally publishedYes

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T-Lymphocytes
T-Lymphocyte Subsets
Transgenic Mice
Glycoproteins
Phenotype
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Tsujimura, K., Obata, Y., Matsudaira, Y., Nishida, K., Akatsuka, Y., Ito, Y., ... Takahashi, T. (2006). Characterization of murine CD160+ CD8+ T lymphocytes. Immunology Letters, 106(1), 48-56. https://doi.org/10.1016/j.imlet.2006.04.006
Tsujimura, Kunio ; Obata, Yuichi ; Matsudaira, Yasue ; Nishida, Keiko ; Akatsuka, Yoshiki ; Ito, Yoshinori ; Demachi-Okamura, Ayako ; Kuzushima, Kiyotaka ; Takahashi, Toshitada. / Characterization of murine CD160+ CD8+ T lymphocytes. In: Immunology Letters. 2006 ; Vol. 106, No. 1. pp. 48-56.
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Tsujimura, K, Obata, Y, Matsudaira, Y, Nishida, K, Akatsuka, Y, Ito, Y, Demachi-Okamura, A, Kuzushima, K & Takahashi, T 2006, 'Characterization of murine CD160+ CD8+ T lymphocytes', Immunology Letters, vol. 106, no. 1, pp. 48-56. https://doi.org/10.1016/j.imlet.2006.04.006

Characterization of murine CD160+ CD8+ T lymphocytes. / Tsujimura, Kunio; Obata, Yuichi; Matsudaira, Yasue; Nishida, Keiko; Akatsuka, Yoshiki; Ito, Yoshinori; Demachi-Okamura, Ayako; Kuzushima, Kiyotaka; Takahashi, Toshitada.

In: Immunology Letters, Vol. 106, No. 1, 15.07.2006, p. 48-56.

Research output: Contribution to journalArticle

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AU - Tsujimura, Kunio

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Tsujimura K, Obata Y, Matsudaira Y, Nishida K, Akatsuka Y, Ito Y et al. Characterization of murine CD160+ CD8+ T lymphocytes. Immunology Letters. 2006 Jul 15;106(1):48-56. https://doi.org/10.1016/j.imlet.2006.04.006